机构地区:[1]四川农业大学动物营养研究所,雅安625014 [2]西藏职业技术学院,拉萨850000
出 处:《动物营养学报》2017年第1期97-105,共9页CHINESE JOURNAL OF ANIMAL NUTRITION
基 金:四川省科技支撑计划(2013NZ0054);四川农业大学双支计划
摘 要:本研究旨在评定棉籽粕在英系北京鸭上的净能(NE),并运用棉籽粕的表观代谢能(AME)和常规成分建立其预测方程。根据析因法将棉籽粕替代饲粮的NE剖分为维持净能(NEm)和生产净能(NEp),利用回归法测定NEm,比较屠宰法测定NEp,再套算得到棉籽粕的NE。选取400只7日龄的英系北京鸭进行动物试验。首先选取20只肉鸭在试验开始时挫颈致死,测定其体能量作为测定NEm和NEp的共同初始能量对照;再选取50只肉鸭用于NEm的测定,NEm用禁食产热(FHP)估计,共设5个组(每组5个重复,每个重复2只),分别为自由采食组及限饲15%、25%、35%、45%组,均饲喂基础饲粮1;剩余的330只肉鸭用于NEp的测定,共设33个组(每组5个重复,每个重复2只),分别饲喂基础饲粮2和以不同棉籽粕替代15%基础饲粮2的试验饲粮。于14日龄时将所有的试鸭挫颈致死,测定所有试鸭的体能量。最后,将实测棉籽粕NE与AME和其常规成分进行相关分析和多元逐步线性回归分析建立棉籽粕NE的预测方程。结果显示:英系北京鸭的FHP为580.7 k J/(kg BW0.75·d),棉籽粕替代饲粮提供给肉鸭的NEm和NEp分别为(2.97±0.15)MJ/kg和(5.22±0.12)MJ/kg,棉籽粕替代饲粮提供给肉鸭的NE为(8.19±0.09)MJ/kg,计算得到的棉籽粕的NE和AME分别为(6.12±0.62)MJ/kg和(10.05±1.02)MJ/kg,AME转化为NE的效率为(60.97±2.04)%,使用AME和常规成分建立棉籽粕NE的最佳预测方程为NE=3.276+0.241AME+0.044CP-0.081ADF[决定系数(R2)=0.954,残余标准差(RSD)=0.13 M J/kg,P<0.01],式中CP为粗蛋白质,ADF为酸性洗涤纤维。由以上结果可知:英系北京鸭棉籽粕的NE为(6.12±0.62)MJ/kg,分布范围为5.04~7.30 M J/kg,变异较大;运用AM E结合常规成分可以准确预测英系北京鸭棉籽粕的NE。The objective of this study was to evaluate the net energy( NE) of cottonseed meal in Cherry Valley Peking ducks,and to establish the prediction models for NE of cottonseed meal using apparent metabolizable energy( AME) and conventional composition. The factorial method was employed to evaluate the NE of cottonseed meal,and the NE of cottonseed meal was divided into NE maintenance( NEm) and NE production( NEp) which measured through regression method and comparative slaughting method,respectively. The NE of cottonseed meal was calculated by its substitution in the diet. In animal experiment,a total of 400 Cherry Valley Peking ducks were selected. Firstly,twenty ducks were selected and killed by cervical dislocation to determine both NEm and NEp according to the initial body energy of ducks at the beginning of the experiment.Secondly,fifty ducks were selected and used to measure NEm which were divided into five groups including ad libitum and 15%,25%,35% and 45% feed restriction groups( each group had 5 replicates and each replicate had 2 ducks),and all ducks were fed basal diet 1. The NEm was estimated by fast heat production( FHP).Thirdly,the other 330 ducks for NEp measure were divided into 33 groups( each group had 5 replicates and each replicate had 2 ducks),and fed basal diet 2 and experimental diets which used different cottonseed meals to replace 15% basal diet 2,respectively. All ducks were killed at 14 days of old by cervical dislocation to determine body energy. Finally,the AME and conventional composition of cottonseed meal were used to conduct related analysis and multiple linear stepwise regression analysis with the measured NE of cottonseed meal,and to establish the prediction models of NE for cottonseed meal. The results of this study showed as follows: the FHP of Cherry Valley Peking ducks was 580.7 k J/( kg BW0.75·d). The NEm and NEp of diet substituted with cottonseed meal were( 2.97 ± 0.15) MJ/kg and( 5.22 ± 0.12) MJ/kg,respectively. The NE of diet s
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