过瘤胃胆碱对黔北麻羊瘤胃发酵及血浆生化指标的影响  

Effects of Rumen-Protected Choline on Rumen Fermentation and Plasma Biochemical Indices of Qianbeima Goats

作　　者：赵鹏[1] 吴文旋[1,2] 吴佳海 田兴舟[1] 莘海亮[4] 袁鑫[1] 杨春红[1] 杨康[1] 孙伦[1] 韩娥[1] 

机构地区：[1]贵州大学动物科学学院,贵阳550025 [2]贵州大学新农村发展研究院,贵阳550025 [3]贵州省草业研究所,贵阳550006 [4]黔东南民族职业技术学院,凯里556000

出　　处：《动物营养学报》2017年第1期333-338,共6页CHINESE JOURNAL OF ANIMAL NUTRITION

基　　金：国家自然科学基金(31360563);贵州山区牧草产业化生产技术研究集成与应用(黔科合重大专项字[2014]6017号);贵州大学科技兴村人才培育基地建设项目;黔东南农作物秸秆加工利用营养价值评定(黔东南科合J字[2016]015号)

摘　　要：本试验旨在研究过瘤胃胆碱(RPC)对成年黔北麻羊瘤胃发酵及血浆生化指标的影响。以体况良好,年龄、体重[(35.0±1.2)kg]一致的成年黔北麻羊6只为试验动物,分为对照组和试验组,每组3个重复,每个重复1只羊。采用交叉试验设计,对照组饲喂基础饲粮,不含RPC;试验组补饲10 g/d的RPC。试验持续30 d,分2期,每期15 d;每期最后1 d采集瘤胃液测定p H、氨态氮浓度、微生物蛋白产量、挥发性脂肪酸(乙酸、丙酸、丁酸)浓度、纤维素酶(微晶纤维素酶、羧甲基纤维素酶、纤维二糖酶、木聚糖酶)活性,同时采血测定血浆葡萄糖、尿素氮、总蛋白、白蛋白、总胆固醇、甘油三酯、肌酐浓度。结果显示:1)RPC对山羊采食速度没有影响,对采食量没有显著影响(P>0.05);2)RPC对瘤胃液p H、氨态氮浓度、微生物蛋白产量、挥发性脂肪酸浓度及4种纤维素酶活性影响较小,2组间差异均不显著(P>0.05);3)RPC对血浆生化指标影响较小,2组间差异均不显著(P>0.05)。由此可见,补饲10 g/d的RPC对成年黔北麻羊的采食量、瘤胃发酵参数和血浆生化指标没有显著影响。The present study was conducted to investigate the effects of rumen-protected choline（ RPC） on rumen fermentation and plasma biochemical indices of Qianbeima goats. Six healthy goats were allocated to 2groups with 3 replicates per group and 1 goat per replicate using a cross-over design. Goats in control group were fed a basal diet,and those in test group were supplemented with 10 g/d RPC. The experiment lasted for30 days including 2 phases with 15-day per phase. Rumen fluid and plasma were collected at the last day of each phase. Rumen fluid p H,ammonia nitrogen concentration,microbial protein yield,volatile fatty acid（ acetic acid,propionic acid and butyric acid） concentrations and cellulase（ microcrystalline cellulose,carboxymethyl cellulose,cellobiase and xylanase） activities were determined; plasma concentrations of glucose,urea nitrogen,total protein,albumin,total cholesterol,triglyceride and creatinine were also determined. The results showed as follows： 1） RPC had no effect on feeding speed,and had no significant effect on feed intake（ P〉0.05）; 2） rumen fluid p H,ammonia nitrogen concentration,microbial protein yield,volatile fatty acid concentrations and 4 kinds of cellulase activities were not significantly affected by RPC supplementation（ P 〉0.05）; 3） there were no significant differences in plasma biochemical indices between control and test group（ P〉0.05）. It is indicated from the above-mentioned results that 10 g/d RPC supplementation has no significant effects on feed intake,rumen fermentation parameters and plasma biochemical indices of Qianbeima goats.

关 键 词：过瘤胃胆碱 瘤胃发酵 血浆生化指标 黔北麻羊 

分 类 号：S816.7[农业科学—饲料科学]