重组腺病毒对高糖诱导的人脐静脉内皮细胞一氧化氮生成的影响及机制  

Effect of Ad-HGF on Production of Nitric Oxide in High Glucose Induced HUVECs and Its Mechanism

作　　者：王娟 徐倩[3] 曹荟哲[2] 曾通旭 白燕青 杨志华 哈小琴[2] WANG Juan XU Qian CAO Hul-zhe ZENG Tong-xu BAI Yan-qing YANG Zhi-hua HA Xiao-qin(Department of Clinical Laboratory, Juye County People＇s Hospital, Juye, Shandong 274900, China the Second Hospital of Lanzhou University, Lanzhou 730000, China Department of Clinical Laboratory, Lanzhou General Hospital of Lanzhou Military Area Command, Lanzhou 730050, China)

机构地区：[1]巨野县人民医院检验科,山东巨野274900 [2]兰州军区兰州总医院检验科,兰州730050 [3]兰州大学第二临床学院,兰州730000

出　　处：《解放军医药杂志》2017年第1期15-19,共5页Medical & Pharmaceutical Journal of Chinese People’s Liberation Army

基　　金：国家自然科学基金(81273568)

摘　　要：目的观察感染携带肝细胞生长因子的重组腺病毒(Ad-HGF)对高糖诱导的原代人脐静脉内皮细胞(human umbilical vein endothelial cells,HUVECs)一氧化氮(NO)生成的影响,并探究其机制。方法将HUVECs分为低糖组(LG组)、高糖组(HG组)、腺病毒对照组(HG+Ad-GFP组)和实验组(HG+Ad-HGF组),感染48 h后,采用NO荧光探针检测细胞内NO水平,蛋白免疫印迹法(Westren Blot)分别检测各组细胞鞘氨醇激酶1(sphingosine kinase1,SPHK1)、沉默信息调节因子2相关酶类1(silent mating type information regulation 2 homolog 1,SIRT1)和内皮型一氧化氮合酶(endothelial ntric oxide synthase,e NOS)蛋白表达。结果 HG组HUVECs细胞内NO水平及SIRT1和e NOS蛋白表达水平低于LG组,HG+Ad-HGF组高于HG组(P<0.05),但4组间SPHK1表达水平比较差异无统计学意义(P>0.05)。结论 Ad-HGF可通过激活SIRT1-e NOS-NO通路保护HUVECs免受高糖损伤。Objective To observe effect of adenovirushepatocyte growth factor （Ad-HGF） on production of nitric oxide （NO） in high glucose （HG） induced human umbilical vein endothelial cells （HUVECs） and its mechanism. Methods HUVECs were divided into low glucose （LG） , HG, HG transfect with Ad-HGF （ HG ＋ Ad- HGF） and HG transfect with Ad- GFP （HG ＋ Ad-GFP） groups. After infection for 48 h, NO levels were detected using NO fluorescent probe; Westren Blot method was used to detect expressions of sphingosine kinase 1 （SPHK1） , silent mating type information regulation 2 homolog 1 （SIRT1） and endothelial nitric oxide synthase （eNOS）. Results NO levels and SIRT1 and eNOS expressions in HUVECs in HG group were lower than those in LG group, while the above values in HG ＋ Ad- HGF group were higher than those in HG group （P 〈 0.05） , but there were no significant differences in SPHK1 expressions among the 4 groups （P 〉 0.05）. Conclusion Ad-HGF can protect HUVECs from high glucose damage by activating SIRTI-eNOS-NO pathway.

关 键 词：重组腺病毒 人脐静脉内皮细胞 鞘氨醇激酶1 沉默信息调节因子2相关酶类1 内皮型一氧化氮合酶 

分 类 号：R587.23[医药卫生—内分泌]