感染Ad-HGF对高糖诱导的人脐静脉内皮细胞Bax和Bcl-2蛋白表达的影响  

Effects of High Glucose Induced HUVECs Infected by Ad-HGF on Bax and Bcl-2 Protein Expressions

作　　者：哈小琴[1] 王娟[1,2] 白燕青 徐倩 曹荟哲[1] 曾通旭 杨志华 HA Xiao-qin WANG Juan BAI Yan-qing XU Qian CAO Hui-zhe ZENG Tong-xu YANG Zhi-hua(Department of Clinical Laboratory, Lanzhou General Hospital of Lanzhou Military Area Command, Lanzhou 730050, China Department of Clinical Laboratory, Juye County People＇s Hospital, Juye, Shandong 274900, China)

机构地区：[1]兰州军区兰州总医院检验科,兰州730050 [2]巨野县人民医院检验科,山东巨野274900

出　　处：《解放军医药杂志》2017年第1期20-23,共4页Medical & Pharmaceutical Journal of Chinese People’s Liberation Army

基　　金：国家自然科学基金(81273568)

摘　　要：目的探讨高糖条件下感染携带肝细胞生长因子的重组腺病毒(Ad-HGF)对人脐静脉内皮细胞(human umbilical vein endothelial cells,HUVECs)凋亡相关蛋白Bax、Bcl-2表达的影响。方法将HUVECs分为低糖组(LG组,5.5 mmol/L)、高糖组(HG组,35 mmol/L)、腺病毒对照组(HG+Ad-GFP组)和实验组(HG+Ad-HGF组)。检测4组HUVECs增殖情况、细胞内活性氧(ROS)水平及凋亡相关蛋白Bax、Bcl-2的表达。结果 HG组、HG+Ad-GFP组HUVECs存活率低于LG组,HG+Ad-HGF组高于HG组(P<0.05,P<0.01)。HG组、HG+Ad-HGF组HUVECs内ROS水平高于LG组,但HG+Ad-HGF组低于HG组(P<0.05)。HG组、HG+Ad-GFP组Bax、Bax/Bcl-2高于LG组,Bcl-2低于LG组,但HG+Ad-HGF组Bax、Bax/Bcl-2低于HG组,Bcl-2高于HG组(P<0.05)。结论感染Ad-HGF可通过降低细胞内ROS水平和Bax/Bcl-2减少细胞凋亡,进而对高糖诱导的HUVECs起到保护作用。Objective To investigate effects of high glucose （HG） induced human umbilical vein endothelial cells （HUVECs） infected by adenovirushepatocyte growth factor （Ad-HGF） on protein expressions of apoptosis related Bax and Bcl-2. Methods HUVECs were divided into low glucose （LG, 5.5mmol/L）, HG （35mmool/L） , HG transfeet with Ad-HGF （ HG ＋ Ad- HGF） and HG transfect with Ad- GFP （ HG ＋ Ad-GFP） groups. Proliferation conditions of HUVECs, reactive oxygen species （ROS） levels and expressions of apoptosis related proteins Bax and Bcl-2 were detec- ted. Results Survival rates of HUVECs in HG ancl HG ＋ Ad-GFP groups, were lower than that in LG group, while the rate in HG＋Ad-GFP group was higher than that in HG group （P〈0.05, P〈0.01）. ROS ]eveisin HG and HG＋Ad- HGF groups were higher than that in LG group, while the level in HG ＋ Ad-HGF group was lower than that in HG group （P 〈 0. 05 ）. Bax and Bax/Bcl-2 expressions were higher, while Bcl-2 expressions were lower in HG and HG ＋ Ad-GFP groups than those in LG group; Bax and Bax/Bcl-2 values in HG ＋ Ad-HGF group were lower than those in HG group （P 〈 O. 05）. Conclusion Transfecting the Ad-HGF can achieve protection for HG induced HUVECs by decreasing the intracellular ROS level and Bax/Bcl-2 apoptosis rate.

关 键 词：重组腺病毒 人脐静脉内皮细胞 BAX Bcl-2 

分 类 号：R587.1[医药卫生—内分泌]