黄芪多糖缓解肉牛短途运输应激的效果研究  

Effect of Astragalus Polysaccharides on Short-distance Transport Stress in Beef Cattle

作　　者：刘延鑫[1,2] 孙宇[2] 李业亮[1] 翟磊[2] 郝万清[1] 高腾云[2] LIU Yan-xin SUN Yu LI Ye-liang ZHAI Lei HAO Wan-qing GAO Teng-yun(Henan University of Traditional Chinese Medicine, Zhengzhou 450008, China College of Animal Husbandry and Veterinary Medicine Engineering, Henan Agricultural University, Zhengzhou 450002, China)

机构地区：[1]河南中医药大学,郑州450008 [2]河南农业大学牧医工程学院,郑州450002

出　　处：《中国畜牧兽医》2017年第1期87-93,共7页China Animal Husbandry & Veterinary Medicine

基　　金：现代农业产业技术体系建设专项资金项目(CARS-37);中国博士后面上基金项目(2012M521396)

摘　　要：为了研究黄芪多糖缓解肉牛短途运输应激的效果,选择15头(12月龄、体重250kg左右)健康西门塔尔杂交肉牛,随机分至对照组、运输应激组和黄芪多糖处理组中,每组5头牛,黄芪多糖处理组牛给予10g/(d·头)的黄芪多糖,运输应激组和黄芪多糖处理组进行3h的60km/h模拟公路运输。运输前1d及运输后1、7d采集所有试验牛的血样,测定相关生化指标,同时统计肉牛日增重和发病率。结果表明,运输应激组肉牛血清Na+在运输后1d显著升高(P<0.05),对照组和黄芪多糖处理组在试验期间各时间点血清Na+、K+、Ca2+和Cl-无显著差异(P>0.05);运输应激组在运输后1、7d的Na+/K+-ATP酶活性均极显著低于运输前1d(P<0.01),血清SOD活性、GSH-Px活性均显著低于运输前1d(P<0.05),运输后1、7d血清MDA含量均显著高于运输前1d(P<0.05),对照组和黄芪多糖处理组上述指标在试验期间均无显著变化(P>0.05);对照组和黄芪多糖处理组运输后7d平均日增重均极显著高于运输应激组(P<0.01),黄芪多糖处理组的呼吸道和消化道发病率均低于运输应激组。综上,黄芪多糖对肉牛短途运输应激防治具有一定的应用价值。To study the effect of Astragaluspolysaccharide on short-distance transport stress in beef cattle,15 healthy and 12-month-old Simmental crossbred beef cattle were selected and randomly divided into control group,transport stress group and Astragalus polysaccharide test group with 5cattles in each group.The cattles in Astragalus polysaccharide test group were fed with10g/d per head Astragalus polysaccharide during the experimental period.The cattles both in transport stress group and Astragalus polysaccharide test group were transported for 3hat the speed of 60km/h,and blood samples were collected and used for measuring some biochemical parameters.The daily gain and morbidity were measured simultaneously.The results showed that,in transport stress group,serum Na~＋increased significantly in 1dafter transportation comparing to before transportation（P〈0.05）.serum Na~＋,K~＋,Ca2~＋and Cl-showed no significant difference during the experimental period in control group and Astragalus polysaccharide test group（P〉0.05）.After transport（1and 7d）,blood Na~＋/K~＋-ATP activities were extremely significantly decreased（P〈0.01）,SOD and GSH-Px activities were significantly decreased（P〈0.05）,while serum MDA contentswere significantly increased（P〈0.05）in transport stress group comparing with that at 1dbefore transport.These indicators of the control and Astragaluspolysaccharide test group showed no significantly difference during the experimental period（P〈0.05）;7daverage daily gain after transportation of control group and Astragalus polysaccharide test group were extremely significantly higher than transport stress group（P〈0.01）,incidence rates of respiratory and gastrointestinal of Astragalus polysaccharide test group was lower than transport stress group.The results demonstrated that Astragaluspolysaccharide could be applied to the transport stress for prevention and treatment in beef cattle.

关 键 词：运输应激 黄芪多糖 肉牛 抗氧化酶 Na+/K+-ATP酶 

分 类 号：S823.92[农业科学—畜牧学]