ESR、OPN和RBP4基因多态性对大白猪繁殖性状的影响  

Influence of ESR,OPN and RBP4 Genes Polymorphisms on Reproductive Traits of Yorkshire Sows

作　　者：刘乙[1] 马力鹏[1] 李涛[1] 李梦寻[1] 沈永巧 黄涛[1] 胡九英 汪德明 王忻 来红霞 LIU Yi MA Li-peng LI Tao LI Meng-xun SHENG Yong-qiao HUANG Tao HU Jiu-ying WANG De-ming WANG Xin LAI Hong-xia(College of Animal Science and Technology , Shihezi University, Shihezi 832000, China Xinjiang Chia Tai Food Co. , Ltd. , Wujiaqu 831301, China)

机构地区：[1]石河子大学动物科技学院,石河子832000 [2]新疆正大食品有限公司,五家渠831301

出　　处：《中国畜牧兽医》2017年第1期123-130,共8页China Animal Husbandry & Veterinary Medicine

基　　金：国家国际科技合作项目(2014DFA31840);国家自然科学基金(31460586);教育部留学归国人员科研启动基金((2013)1092);石河子大学动植物育种专项(gxjs2013-yz06)

摘　　要：为了探讨雌激素受体(estrogen receptor,ESR)、骨桥蛋白(osteopontin,OPN)和视黄醇结合蛋白4(retinol binding proteins 4,RBP4)的多态性对大白猪繁殖性状的影响,本研究采用PCR-RFLP技术检测试验猪群ESR、OPN和RBP4基因的多态性,并分析其对大白猪总产仔数、产活仔数、健仔数、窝重、初生重、死胎、木乃伊、弱仔数和畸形数等性状的影响。结果显示,在大白经产母猪中,ESR和OPN基因的优势基因为A等位基因,RBP4基因的优势基因为B等位基因;ESR基因AB与BB基因型在总产仔数、产活仔数性状上均高于AA基因型;OPN基因在总产仔数性状上AA基因型最高,在产活仔数、健仔数、窝重性状上均为BB>AA>AB;RBP4基因在总产仔数、产活仔数、健仔数、窝重性状上均为AA>BB>AB;合并基因型分析发现ESR-RBP4最优的分子标记组合为ABAA;OPN-RBP4最优的分子标记组合为BBAA。因此,ESR、OPN和RBP4基因多态性与大白猪繁殖性状相关。To study the influence of estrogen receptor （ESR）, osteopontin （OPN） and retinol binding proteins 4 （RBP4） genotypes on reproductive performance of Yorkshire sows, PCR- RLFP technique was used for genotyping, and the effects of genotypes on reproductive traits were analyzed. The results showed that A allele of ESR and OPN genes were predominant allele, and B allele of RBP4 gene was predominant allele. TNB, NBA of ESR gene AB and BB genotypes were higher than AA genotype;TNB of OPN gene AA genotype was the highest, but the NBA, NHB and WB for OPN gene genotypes were BB〉AA〉AB;The TNB,NBA,NHB and WB for RBP4 gene genotypes were AA〉BB〉AB. Then combing genotype analysis showed that ABAA of ESR-RBP4 and BBAA of OPN-RBP4 were the most prolific alleles, respectively. The present data revealed that ESR, OPN and RBP4 genes polymorphisms were associated with reproductive performance of Yorkshire sows.

关 键 词：ESR基因 OPN基因 RBP4基因 母猪 繁殖性状 

分 类 号：S814.8[农业科学—畜牧学]