版纳微型猪近交系精子冷冻保存研究  

Study of Semen Cryopreservation for Banna Mini-pig Inbred Line

作　　者：查星琴[1,2] 肖晶[2] 成文敏[1,2] 霍金龙[1,2] 王淑燕[1,2] 王配[1,2] 潘伟荣[1,2] 曾养志[1] ZHA Xing-qin XIAO Jing CHENG Wen-min HUO Jin-long WANG Shu-yan WANG Pei PAN Wei-rong ZENG Yang-zhi(Key Laboratory of Banna Mini-pig Inbred Line of Yunnan Provice, Yunnan Agricultural University, Kunming 650201, China College of Animal Sciences and Technology , Yunnan Agricultural University, Kunming 650201, China)

机构地区：[1]云南农业大学云南省版纳微型猪近交系重点实验室,昆明650201 [2]云南农业大学动物科学技术学院,昆明650201

出　　处：《中国畜牧兽医》2017年第1期167-172,共6页China Animal Husbandry & Veterinary Medicine

基　　金：云南省教育厅科学研究基金项目(2014Y212)

摘　　要：在版纳微型猪近交系(BMI)公猪精液冷冻保存稀释液中添加不同浓度甘油,并对不同解冻液配方和解冻方法进行研究,对比解冻后的精子活力、质膜完整率和顶体完整率,优化BMI公猪精液冷冻保存方法。结果表明,解冻后,甘油浓度为2%和3%组的精子活力、质膜完整率、顶体完整率显著高于1%、4%和5%组(P<0.05);Ⅰ号解冻液解冻后精子活力、质膜完整率、顶体完整率均显著高于Ⅱ、Ⅲ和Ⅳ号解冻液(P<0.05);在活力、质膜完整率和顶体完整率方面,40℃6s和50℃6s这两种程序的解冻效果都显著优于38℃30s(P<0.05)。由此可见,2%或3%的甘油作为抗冻保护剂、Ⅰ号解冻液解冻、40℃6s或50℃6s水浴解冻是较为理想的BMI公猪精液冷冻保存方法。In this study,different concentrations of glycerol were added to semen cryoprotective extenders of Banna Mini-pig inbred line（BMI）,different thawing solutions and thawing methods were studied.After thawing,the motility,the rates of plasma integrity and acrosome integrity were compared to optimize the cryopreservation method of BMI semen.The results showed that the motility,the rates of plasma integrity and acrosome integrity were significantly higher in 2%and 3% glycerol groups than those in 1%,4%and 5% glycerol groups（P〈0.05）;The motility,the rates of plasma integrity and acrosome integrity were significantly higher in thawing solutionⅠthan those in thawing solutionⅡ,ⅢandⅣ（P〈0.05）;Through comparing the motility,the rates of plasma integrity and acrosome integrity,the thawing effect in 40 ℃ 6sand 50 ℃ 6s groups were better than that in 38℃30s（P〈0.05）.In conclusion,the optimal semen cryoprotective method for BMI was 2% or 3% glycerol as cryoprotectant,using thawing solution Ⅰ to thaw at 40 ℃ 6sor 50 ℃ 6s.

关 键 词：版纳微型猪近交系 猪精液 冷冻保存 解冻液 

分 类 号：S814.8[农业科学—畜牧学]