犊牛轮状病毒和大肠杆菌混合感染检测  

The Test of Polyinfection of Bovine Rotavirus and Escherichia coli

作　　者：李艳婷[1] 孙光野 张露[1] 王珊珊[1] 高国强[1] 侯喜林[1] LI Yan-ting SUN Guang-ye ZHANG Lu WANG Shan-shan GAO Guo-qiang HOU Xi-lin(College of Animal Science and Veterinary Medicine, Heilongjiang Bayi Agricultural University, Daqing 163319, China)

机构地区：[1]黑龙江八一农垦大学动物科技学院,大庆163319

出　　处：《中国畜牧兽医》2017年第1期248-253,共6页China Animal Husbandry & Veterinary Medicine

基　　金：国家科技支撑计划课题(2012BAD12B05-2)

摘　　要：黑龙江省某规模化奶牛场部分新生犊牛发生了严重腹泻且犊牛病死率较高。为了确诊,无菌采集病料,通过病毒检测、细菌分离、生化试验、细菌和病毒特异性基因PCR扩增、动物致病性试验及药敏试验等方法进行了病毒抗原和微生物检测。结果显示,病毒检测中牛轮状病毒(BRV)呈阳性,PCR扩增得到400bp的特异性片段;从病料中分离到1株含K99和F41兼性菌毛抗原的高致病性产肠毒素大肠杆菌(ETEC),该分离菌株对恩诺沙星、头孢唑啉、氯霉素敏感,对其他抗生素有一定的耐药性。流行情况调查结合病原学检查确诊该牛场新生犊牛严重腹泻是由BRV和大肠杆菌混合感染造成的,根据诊断结果进行了针对性地防制,取得了良好的防控效果。Newborn calves in a dairy farms suffered from severe diarrhea disease with high mortality in Heilongjiang province.In order to make a definite diagnosis,the pathological samples were collected aseptically and the viral antigens and microorganism were detected by virus detection,bacteria separation,biochemical test,PCR for the specific gene of virus and bacteria,animal pathogenicity test and antimicrobial susceptibility test.The results showed that the virus detection of bovine rotavirus was positive and specific gene fragment of bovine rotavirus was amplified.One E.coli strain with K99 and F41adhesin antigens which belonged to highly pathogenic enterotoxigenic E.coli was isolated.This strain was sensitive to enrofloxacin,cefamedin and chloramphenicol,however,it showed resistance for other antibiotics.A polyinfection of bovine rotavirus and E.coli were eventually confirmed through epidemic investigation and pathological diagnosis.It had significant preventive effect by taking effective measures according to the diagnosis results.

关 键 词：轮状病毒 大肠杆菌 混合感染 分离鉴定 药敏试验 PCR检测 

分 类 号：S858.23[农业科学—临床兽医学]