196份葡萄属(Vitis L.)种质资源耐热性评价  

Evaluation of 196 Vitis L. Germplasm Resources to Heat Tolerance

作　　者：姜建福[1] 马寅峰[1] 樊秀彩[1] 张颖[1] 孙海生[1] 王利军[2] 刘崇怀[1] JIANG Jian-fu MA Yin-feng FAN Xiu-cai ZHANG Ying SUN Hai-sheng WANG Li-jun LIU Chong-huai(Zhengzhou Fruit Research Institute, Chinese Academy of Agricultural Sciences, Zhengzhou 450009 Institute of Botany, Chinese Academy of Sciences, Beijing 100093)

机构地区：[1]中国农业科学院郑州果树研究所,郑州450009 [2]中国科学院植物研究所,北京100093

出　　处：《植物遗传资源学报》2017年第1期70-79,共10页Journal of Plant Genetic Resources

基　　金：现代农业产业技术体系建设专项(CARS-30-yz-1);中美科技合作项目(2014DFG31860);中国农业科学院科技创新工程专项经费项目(CAAS-ASTIP-2016-ZFRI)

摘　　要：以国家果树种质郑州葡萄圃保存的196份代表性葡萄属种质为试材,利用叶绿素荧光参数法对其耐热性进行了鉴定评价。结果表明:196份葡萄属植物的耐热性叶绿素荧光参数Fv/Fm数值在0.0792~0.6836之间,品种间抗性存在差异,耐热性服从正态分布,表现为多基因控制的数量性状。利用有序样品最优分割聚类法,将其耐热性分为弱、中、强3种类型,叶绿素荧光参数分级阈值分别对应≤0.3、(0.3,0.5]、>0.5,利用该分级标准筛选出了腺枝葡萄双溪03、刺葡萄梅岭山1301、菱叶葡萄0945和和田绿葡萄等48份耐热性强的葡萄种质,起源于我国的野生葡萄耐热性整体上高于其他类群或品种。研究结果对于利用抗性亲本选育耐热葡萄新品种和葡萄抗热机理研究具有参考意义。Heat tolerance was evaluated for 196 Vitis L. accessions from National Grape Resources Repository at Zhengzhou Fruit Research Institute of CAAS using the chlorophyll fluorescence parameter Fv/Fm. The results showed that the range of the Fv/Fm was from 0. 0792 to 0. 6836, displaying differences among the varieties, with the distribution frequency of all heat tolerance Fv/Fm in normal distribution. It represents a quantitative trait controlled by multiple genes. Based on sequential clustering method, the grade of grape heat tolerance could be divided into 3 levels,weak, medium and strong. The corresponding threshold value were ≤0. 3, （0. 3,0. 5 ] and 〉 0. 5, respectively. Based on the above evaluation, 48 heat-resistant grape accessions were screened out, including Shuangxixianzhi03, Meilingshan-eiputao1301, Lingyeputao0945 and Hetianlv. The heat tolerance of Chinese wild grape species was in general higher than that of other Vitis L. species / varieties. The result from this study provide valuable insights for breeding heat tolerant grapes and for studying the mechanisms of grape heat resistance.

关 键 词：葡萄属 耐热性 评价 叶绿素荧光 

分 类 号：S663.1[农业科学—果树学]