小半夏加茯苓醇提物对HepG2、BGC823线粒体凋亡途径的影响  

Impacts of small Banxia plus fuling ethanol extract on mitochondrial apoptotic pathway of HepG2 and BGC823

作　　者：贾亚玲 何前松[2] 冯泳[3] 闫文娟[3] 陈丽丽[3] 罗俊刚 

机构地区：[1]贵州省铜仁市万山区人民医院中医科,554200 [2]贵阳中医学院第二附属医院神经内科,贵阳550002 [3]贵阳中医学院药学院,贵阳550002

出　　处：《重庆医学》2017年第3期296-298,301,共4页Chongqing medicine

基　　金：国家自然科学基金项目(81160425/H2705);贵阳中医学院研究生教育创新项目(ZYY14034)

摘　　要：目的探讨小半夏加茯苓醇提物对人肝癌细胞HepG2和人胃腺癌细胞BGC823增殖抑制作用及线粒体凋亡途径的影响。方法运用CCK-8法检测HepG2、BGC823的增殖抑制情况,计算生长抑制率及半数抑制浓度;运用流式细胞术和实时荧光定量PCR检测HepG2、BGC823的线粒体跨膜电位和bax、cjun基因的表达情况。结果小半夏加茯苓醇提物对HepG2、BGC823均有显著的抑制作用(P<0.01),其半数抑制浓度分别为(781.50±13.00)μg/mL和(560.05±10.06)μg/mL;与空白对照组比,试验组HepG2的荧光强度升高(P<0.05),试验组BGC823的荧光强度显著升高(P<0.01);HepG2、BGC823试验组的bax、cjun基因的相对表达量与空白对照组比较,均显著升高(P<0.01)。结论小半夏加茯苓醇提物诱导HepG2、BGC823凋亡的机制可能与激活线粒体途径有关。Objective To investigate the inhibition effect of small Banxia plus Fuling ethanol extract on proliferation of human liver cancer cells HepG2 and human gastric cancer cells BGC823 and its influence on mitochondrial apoptotic pathway.Methods CCK-8was used to test the growth inhibition of HepG2 and BGC823.The growth inhibition rate and the half inhibitory concentration（IC50）of HepG2 and BGC823were calculated;the flow cytometry and real-time fluorescence quantitative PCR were adopted to detect the mitochondrial transmembrane potential and the expression of cjun and bax genes of HepG2 and BGC823.Results HepG2 and BGC823are significantly inhibited by small Banxia plus fuling ethanol extract（P〈0.01）,IC50 was（781.50±13.00）μg/mL and（560.05±10.06）μg/mL respectively.Compared with the blank control group,the fluorescence intensity of HepG2 in the experimental group was increased（P〈0.05）,and which of BGC823 was increased more obviously（P〈0.01）;compared with those of the blank control group,the bax and cjun genes relative expressions in experimental group of HepG2 and BGC823were significantly increased（P〈0.01）.Conclusion The mechanism of small Banxia plus Fuling ethanol extract inducing HepG2 and BGC823apoptosis may be related to activate the mitochondrial pathway.

关 键 词：半夏属 茯苓 肝肿瘤 胃肿瘤 小半夏加茯苓醇提物 肝癌细胞HepG2 胃腺癌细胞BGC823 线粒体凋亡途径 

分 类 号：R285[医药卫生—中药学]