原发性高血压患者内源性H_2S变化及其与IL-6相关性分析  

Analysis on correlation between endogenous H_2S change and IL-6

作　　者：徐言俊 孟宪香 张霞 彭福强 任永生[2] 

机构地区：[1]广东省佛山市南海区第三人民医院急诊科,528200 [2]湖北医药学院生理教研室,湖北十堰442000

出　　处：《重庆医学》2017年第3期341-343,共3页Chongqing medicine

基　　金：广东省佛山市医学类科技攻关项目(2015AB000762)

摘　　要：目的探讨原发性高血压(EH)发病中H2S的变化及其与炎症因子IL-6的相关性。方法收集46例健康对照(正常对照组)和80例EH患者血浆,将EH患者分为药物控制组(n=47)和未使用药物控制组(n=33);根据舒张压值将EH患者分为A组(舒张压<90mm Hg,n=21)、B组(90~<100mm Hg,n=23)、C组(100~110mm Hg,n=18)及D组(>110mm Hg,n=18)。采用亚甲基蓝法测定血清H2S,双抗体夹心酶联免疫吸附试验(ELISA)检测血浆IL-6的浓度。结果 EH患者及A、B、C、D组血浆H2S水平[(8.50±0.59)、(8.94±0.62)、(8.64±0.52)、(8.20±0.41)、(8.23±0.46)μmol/L],均显著低于正常对照组[(9.29±0.91)μmol/L,P<0.01]。与EH患者未使用药物控制组比较,药物控制组血浆H2S水平显著增加[(8.37±0.55)μmol/L vs.(8.77±0.53)μmol/L],差异有统计学意义(P<0.01);与正常对照组比较,EH组患者血浆IL-6水平显著增加[(0.48±0.07)ng/L vs.(0.65±0.13)ng/L],差异有统计学意义(P<0.01)。EH患者使用药物控制组血浆IL-6水平较未使用药物控制组显著降低[(0.56±0.07)ng/L vs.(0.71±0.13)ng/L],差异有统计学意义(P<0.01)。EH患者血浆H2S浓度与脉压差呈正相关(r=0.42,P<0.01),与IL-6呈负相关(r=-0.38,P<0.01),与收缩压和舒张压无相关性(P>0.05)。结论 EH患者血浆H2S水平明显降低,且随着舒张压增加而降低,血浆H2S水平与IL-6显著负相关。EH患者血浆H2S可能参与了炎症诱导的EH发病过程。Objective To explore the correlation between the change of plasma hydrogen sulfide（H2S）level and interleukin-6（IL-6）in the pathogenesis essential hypertension（EH）.Methods Plasma was collected from 46 normal controls（control group）and 80 patients with EH.The EH patients were divided into the medication control group（n=47）and non-medication control group（n=33）.According to the diastolic pressure（DP）value,the EH patients were divided into the group A（DP90mm Hg,n=21）,B（DP=90-100mm Hg,n=23）,C（DP=100-110 mm Hg,n=18）and D（DP110mmHg,n=18）.The methylene blue method was adopted to measure serum H2S level,and the plasma IL-6level was detected by using the double antibody sandwich ELISA.Results The plasma H2S level in the EH patients,group A,B,C and D were（8.50±0.59）μmol/L,（8.94±0.62）μmol/L,（8.64±0.52）μmol/L,（8.20±0.41）μmol/L and（8.23±0.46）μmol/L respectively,which were significant lower than（9.29±0.91μmol/L）in the control group（P〈0.01）.Compared with the non-medication control group,the plasma H2S level in the medication control group was significantly increased,the difference was statistically significant[（8.37±0.55）μmol/L vs.（8.77±0.53）μmol/L,P〈0.01];compared with the control group,the plasma H2S level in the EH group was significantly increased,the difference was statistically significant[（8.37±0.55）μmol/Lvs.（8.77±0.53）μmol/L,P〈0.01].Compared with the control group,the plasma IL-6level in the EH group was significantly increased,the difference was statistically significant[（0.48±0.07）ng/L vs.（0.65±0.13）ng/L,P〈0.01];Compared with the non-medication control group,the plasma IL-6level in the medication control group was significantly decreased,the difference was statistically significant[（0.56±0.07）ng/Lvs.（0.71±0.13）ng/L,P〈0.01].Furthermore,the plasma H2S level had significantly positive correlation with the pulse pressure（R=0.42,P〈0.01）,significantly negative correlation with the IL

关 键 词：硫化氢 白细胞介素6 原发性高血压 

分 类 号：R544.11[医药卫生—心血管疾病]