HBV感染者IL-4水平变化及与临床的相关性  

Changes and clinical correlation of serum IL-4 level for patients with chronic hepatitis B

作　　者：李彩东[1] 杨勇卫[1] 陈锡莲[1] 田鹏飞[1] 段正军[1] 刘学梅[1] LI Caidong YANG Yongwei CHEN Xilian TIAN Pengf ei DUAN Zhengjun LIU Xuemei(Liver Diseases Research Institute ,Lanzhou Municipal Second Peoplets Hospital ,Lanzhou 730046 ,Gansu,Chin)

机构地区：[1]甘肃省兰州市第二人民医院肝病研究所,730046

出　　处：《国际检验医学杂志》2017年第2期177-178,181,共3页International Journal of Laboratory Medicine

基　　金：甘肃省兰州市科技计划资助项目(2015-2-7)

摘　　要：目的探讨乙型肝炎病毒(HBV)感染者外周血白细胞介素4(IL-4)水平变化及与临床的相关性。方法收集60例HBV携带者(ASC组)、60例慢性乙型肝炎患者(CHB组)、60例乙型肝炎肝硬化患者(LC组)、60例原发性肝癌患者(HCC组)和50例健康对照者(健康对照组)空腹血清,采用酶联免疫吸附试验(ELISA法)检测血清IL-4水平;采用荧光定量聚合酶链反应(PCR法)检测血清HBV DNA载量;应用全自动生化分析仪检测血清肝功能水平。结果与健康对照组[(1.64±0.17)ng/mL]比较,HBV感染者CHB组、LC组和HCC组外周血IL-4水平均显著升高[(4.18±0.48)、(4.71±0.42)、(3.62±0.31)ng/mL,P<0.05],其中LC组最高,ASC组最低;与ASC组相比,CHB组、LC组和HCC组外周血IL-4水平均显著升高(P<0.05);与HCC组相比,LC组外周血IL-4水平均显著升高(P<0.05);LC组患者IL-4水平与总胆红素(TBIL)水平呈正相关(r=0.529,P<0.01);HCC组患者IL-4水平与丙氨酸氨基转移酶(ALT)和TBIL水平均呈正相关(r=0.263、0.323,P<0.05)。结论 IL-4在HBV发生、发展过程中可能起重要作用,检测其外周血水平可作为评估慢性乙型肝炎病情严重程度的重要指标。Objective To investigate the changes and clinical correlation of IL-4in patients with HBV infection.Methods Sixty cases with chronic asymptomatic HBV carriers,60 cases with chronic hepatitis B,60 cases with liver cirrhosis,60 cases with hepatocellular carcinoma and 50 healthy controls were collected for serum.ELISA was used for detection of cytokine IL-4levels;The amount of HBV DNA was measured by fluorescence quantitative PCR;Liver function was tested by automatic biochemical analyzer.Results Compared with the healthy controls[（1.64±0.17）ng/mL],IL-4levels of patients with CHB,LC and HCC were significantly increased[（4.18±0.48）,（4.71±0.42）,（3.62±0.31）ng/mL,P〈0.05].LC group have the highest IL-4levels,while ASC was the lowest.Compared with the ASC group,IL-4levels of patients with CHB,LC and HCC were significantly increased（P〈0.05）.Compared with the HCC group,IL-4levels of patients with LC were significantly increased（P〈0.05）.IL-4level was positively correlated with TBIL levels in LC patients（r=0.529,P〈0.01）,while the IL-4level was positively correlated with ALT and TBIL level in HCC patients（r=0.263,0.323,P〈0.05）.Conclusion IL-4may play an important role in chronic HBV infection,the levels of IL-4can be used as an important indicator to assess the severity of chronic hepatitis B.

关 键 词：病毒性乙型肝炎 白介素-4 HBV DNA 临床相关性 

分 类 号：R512.62[医药卫生—内科学]