中药筋脉通对糖尿病大鼠背根神经节硝基酪氨酸和神经生长因子表达的影响  

Effects of Chinese Medicinal Compound Jinmaitong on the Expression of Nitrotyrosine and Nerve Growth Factor in the Dorsal Root Ganglia of Diabetic Rats

作　　者：吴亚楠[1] 梁晓春[1] 杨丹[1] 屈岭[1] 刘伟[1] 高云周[1] WU Ya-nan LIANG Xiao-chun YANG Dan Qu Ling LIU Wei GAO Yun-zhou(Department of TCM, PUMC Hospital, CAMS and PUMC, Beijing 100730, China)

机构地区：[1]中国医学科学院北京协和医学院北京协和医院中医科,北京100730

出　　处：《中国医学科学院学报》2016年第5期507-513,共7页Acta Academiae Medicinae Sinicae

基　　金：北京市自然科学基金(7122147)~~

摘　　要：目的观察中药复方筋脉通(JMT)对糖尿病大鼠背根神经节(DRG)硝基酪氨酸(NT)和神经生长因子(NGF)表达的影响。方法采用链脲佐菌素一次性腹腔内注射方法造模,随机数字表法将大鼠分为正常对照组(Con组)、糖尿病模型对照组(DM组)、JMT治疗组(JMT组)及牛磺酸治疗组(Tau组)4组,每组10只。成模后给予灌胃给药,JMT组[1.31 g/(kg·d),给药剂量为生药量]及Tau组[1.20 g/(kg·d)]均按成人剂量15倍给药,DM组和Con组灌服蒸溜水l ml/(100 g·d)。分别于治疗前及治疗后4、8、12、16周检测各组大鼠体重和血糖变化。所有大鼠均于灌胃16周后进行机械痛阈检测后处死,取大鼠DRG组织,采用免疫组织化学染色法和Western blot法检测大鼠DRG中NT及NGF的表达。结果免疫组织化学检测结果显示,与Con组相比,DM组的NT表达显著升高(P=0.000),NGF的表达显著降低(P=0.006);与DM组相比,JMT组及Tau组的NT表达显著降低(P=0.000,P=0.000),NGF表达显著升高(P=0.000,P=0.004),其中JMT治疗组NT较Tau组更为显著(P=0.004)。Western blot检测结果显示,与Con组相比,DM组的NT蛋白表达显著升高(P=0.000),NGF蛋白表达显著降低(P=0.000);与DM组比较,JMT组与Tau组的NT蛋白表达均显著降低(P=0.001,P=0.000),NGF蛋白表达均显著升高(P=0.000,P=0.001)。结论中药JMT能够增加糖尿病大鼠DRG组织中NGF的表达,同时降低NT水平。Objective To study the effects of Chinese medicinal compound Jinmaitong（ JMT） on the expressions of nitrotyrosine（ NT） and nerve growth factor（ NGF） in dorsal root ganglia of diabetic rats.Methods Experimental rat diabetic models were established by the intraperitoneal injection of streptozotocin.Rat models were then randomly divided into four groups including normal control group（ Con group）, diabetes mellitus group（ DM group）,Jinmaitong group（ JMT group）（ treated with JMT similar to the fifteen-fold dose of adult recommended dosage）,and taurine group（ Tau group）（ treated with Taurine similar to the fifteen-fold dose of adultrecommended dosage）,with 10 rats in each group.The Con and DM groups were treated with distilled water at a daily dose of 1 ml/100 g.All rats were given intragastric administration for 16 weeks and then killed.Body weight and blood glucose were detected before and at the 4th,8th,12 th,and 16 th week after treatment.The pain threshold to mechanical stimulation with von Frey filament were carried out before death.The expressions of NT and NGF in dorsal root ganglion were detected by immunohistochemistry and Western blot analysis,respectively.Results Immunohistochemistry showed that the average optical density（ AOD） of NT expression in DM group were significantly higher than those in control group（ P = 0.000）,and the AOD of NGF was significantly lower than the control group（ P = 0.006）.The AOD of NT（ P = 0.000,P = 0.000） in both treatment groups decreased significantly and the AOD of NGF（ P = 0.000,P = 0.004） significantly increased compared with DM group.The AOD of NT in JMT group was significantly lower than Tau group（ P = 0.004）.Western blot analysis showed that the protein level of NT in DM group was significantly higher than that in control group（ P = 0.000）,and the protein level of NGF was significantly lower than that in control group（ P = 0.000）.Compared with the DM group,the protein level of NT in both treatment groups

关 键 词：糖尿病周围神经病变 背根神经节 免疫组织化学 WESTERN BLOT检测 硝基酪氨酸 神经生长因子 筋脉通 

分 类 号：R445.2[医药卫生—影像医学与核医学]