norD对耐甲氧西林金黄色葡萄球菌生物膜形成的影响  

The effects of norDon the formation of biofilm of methicillin-resistant Staphylococcus aureus

作　　者：张智玮[1] 乔林爽 赵月辉[1] 崔洪波[1] 赵吉子[1,2,3] 张文莉[1,2,3] 张凤民[1,2,3] 付英梅[1,2,3] 

机构地区：[1]哈尔滨医科大学微生物教研室,黑龙江哈尔滨150081 [2]哈尔滨医科大学伍连德研究所,黑龙江哈尔滨150081 [3]黑龙江省感染与免疫重点实验室,黑龙江哈尔滨150081

出　　处：《中国微生态学杂志》2016年第12期1379-1381,共3页Chinese Journal of Microecology

基　　金：国家自然科学基金(31370164)

摘　　要：目的探究norD编码的超家族转运蛋白NorD对耐甲氧西林金黄色葡萄球菌生物膜形成的影响。方法使用耐甲氧西林金黄色葡萄球菌MW2野生株和norD基因缺失株及norD互补菌株,微孔法培养形成生物膜,同时使用番红染剂对形成的生物膜染色,通过酶标仪检测特定波长下的A值,对细菌生物膜形成定量分析。结果常规培养时,MW2野生株和MW2 norD缺失株生物膜的形成比较差异无统计学意义(P>0.05)。低Fe^(2+)离子环境下,MW2 norD基因缺失株培养48h,生物膜形成的量少于MW2(t=3.878,P=0.0082)。结论MW2 norD基因缺失株在低Fe^(2+)离子环境下引发生物膜分解,提示NorD可促进Fe^(2+)离子环境下生物膜的形成。Objective To explore the effect of major facilitator superfamily NorD on the formation of biofilm of MRSA strain MW2. Methods The biofilm of MW2 was cultivated through norD over expression and deletion in the 96-well plates, and stained with safranine, and then quantitatively analyzed by detecting A490 value. Results There was no statistical difference （P〉0.05） in the formation of biofilm among MW2, MW2 norD deletion strains, MW2 norD deletion complementary strains and MW2 norD deletion strains containing empty plasmid when cultivated in normal TSB culture. After 24 hours, the amount of biofilm began to decrease. In low Fe2＋ ion environment, the amount of biofilm of MW2 AnorD and the wild strain did not change significantly at 24 hours, while in the following 24 hours the amount of biofilm of MW2 AnorD was less than that of MW2 （P〈0. 001） . Conclusion Biofilm dispersal is triggered in norD deletion strain of MW2 under low Fe2＋ ion condition, suggesting that NorD can promote the formation of biofilm in Fe2＋ ion environment.

关 键 词：生物膜 MRSA norD基因 

分 类 号：R378.11[医药卫生—病原生物学]