茜素红S对中华倒刺鲃幼鱼不同组织抗氧化酶活性和脂质过氧化的影响  

PRELIMINARY STUDY OF THE ALIZARIN RED S EFFECT ON ANTIOXIDANT ENZYMES ACTIVITY AND LIPID PEROXIDATION OF SPINIBARBUS SINENSIS JUVENILES

作　　者：吴金明[1] 霍来江 杜浩[1] 辛苗苗[1,3] 张磊[1,2] 危起伟[1,2] WU Jin-Ming HUO Lai-Jiang DU Hao XIN Miao-Miao ZHANG Lei WEI Qi-Wei(Key Laboratory of Freshwater Biodiversity Conservation and Utilization, Ministry of Agriculture of China, Yangtze River Fisheries Research Institute, Chinese Academy of Fishery Sciences, Wuhan 430223, China Fisheries College of Huazhong Agricultural University, Wuhan 430070, China School of Life Science, Southwest University, Chongqing 400715, China)

机构地区：[1]中国水产科学研究院长江水产研究所,农业部淡水生物多样性保护重点实验室,武汉430223 [2]华中农业大学水产学院,武汉430070 [3]西南大学生命科学学院,重庆400715

出　　处：《水生生物学报》2016年第6期1172-1177,共6页Acta Hydrobiologica Sinica

基　　金：公益性行业(农业)科研专项(No.201203086);长江上游珍稀特有鱼类国家级自然保护区生活环境补偿项目(No.0701979)资助~~

摘　　要：以中华倒刺鲃幼鱼[平均体长(12.3±0.6)cm,平均体重(41.8±3.6)g]为研究对象,通过测定不同浓度茜素红S溶液(ARS)浸泡24h后肝脏、脑和鳃组织中抗氧化酶(SOD、CAT、GSH-Px)的活性和丙二醛(MDA)含量,评估ARS对中华倒刺鲃幼鱼的生理生化影响。结果表明:除脑组织GSH-Px随着ARS浓度的升高而不断升高外,肝脏、鳃和脑组织的抗氧化酶活性均表现为低浓度被诱导而高浓度受抑制的规律,与ARS浓度呈抛物线型剂量效应关系。肝脏、鳃、脑组织抗氧化酶活性达到最大值所对应的浓度分别是300、300和400 mg/L。除鳃部MDA含量随着茜素红S浓度升高不断升高外,脑和肝脏组织的丙二醛含量随着ARS浓度的升高呈现出先降低后上升趋势。根据试验的结果,推荐120—200 mg/L为ARS染色标记中华倒刺鲃幼鱼(体长10 cm)的适宜浓度。To assess the physiological and biochemical effect of Alizarin red S（ARS） on Spinibarbus sinensis juveniles, antioxidant enzymes（SOD, CAT, GSH-Px） activity and malondialdehyde（MDA） content of liver, brain and gill tissue were detected after 24 h treatment in ARS. The results showed that brain GSH-Px activity increased continuously with rising concentration of ARS, other antioxidant enzymes activities in brain and antioxidant enzymes activities in both gill and liver were increased firstly and then decreased, which showed a parabolic dose-response relationship with the ARS concentration. Antioxidant enzyme activity in liver, gills and brain reached to the maximum at the concentration of 300, 300 and 400 mg/L, respectively. The MDA content of brain and liver firstly decreased and then increased with the rising concentration of ARS, but gill MDA content was increased continuously. These results suggest120—200 mg/L of ARS for marking S. sinensis juveniles.

关 键 词：茜素红S 中华倒刺鲃幼鱼 抗氧化酶 丙二醛 

分 类 号：Q142[生物学—生态学]