苦瓜总皂苷对2型糖尿病大鼠心脏组织中MMP-9和HO-1表达影响  

Effect of Total Saponins of Bitter Gourd on MMP-9 and HO-1 in the Heart Tissue of Type 2 Diabetic Rats

作　　者：朱定君[1,2] 刘紫燕[1] 殷旭光[1] 王丽媛[1,2] 

机构地区：[1]黄石市中心医院,湖北黄石435000 [2]湖北理工学院附属医院,湖北黄石435000

出　　处：《辽宁中医药大学学报》2017年第1期48-51,共4页Journal of Liaoning University of Traditional Chinese Medicine

基　　金：湖北省卫生厅资助项目(2014FFA021)

摘　　要：目的:探讨苦瓜总皂苷对2型糖尿病大鼠心肌组织中血红素加氧酶1(Heme Oxygenase1,HO-1)和基质金属蛋白酶-9(Matrix Metallo Protein-9,MMP-9)表达的影响。方法:将60只健康雄性SD大鼠,随机分为正常组、模型组以及苦瓜总皂苷治疗组,模型组和苦瓜总皂苷治疗组给予高脂饮食4周,腹腔一次性注射STZ 30 mg建立2型糖尿病大鼠模型,造模成功后,治疗组给予苦瓜总皂苷(20 mg/kg)灌胃治疗,其它两组给予生理盐水灌胃,分别于治疗4周和8周后处死大鼠各半,检测血糖、心功能以及心肌组织HO-1和MMP-9的表达。结果:与正常对照组相比,模型组血糖,心功能CK、CK-MB、LDH和HBDH明显升高(P<0.05),治疗4周后均明显降低(P<0.05),治疗8周后进一步降低。心肌组织HO-1和MMP-9免疫组化、Western以及RT-PCR可见,与正常组相比糖尿病组HO-1表达明显下降(P<0.05),MMP-9明显升高(P<0.05);治疗后HO-1表达明显增加(P<0.05),MMP-9表达明显减少(P<0.05)。结论:苦瓜总皂苷对大鼠心肌组织中MMP-9和HO-1有明显的调控作用。Objective：To study the effect of the total saponins of bitter gourd on heme oxygenase-1（HO-1）and matrix metallo protein-9（MMP-9）in the heart of D2 TM. Methods：60 male SD rats were randomly divided into normal control group,model control group and total saponins treatment group,the model control group and the total saponins treatment group weregiven high fat diet for 4 weeks,after injection STZ 30 mg to built the type 2 diabetic rat model. Then bitter gourd group was given saponins（20 mg/kg gavage treatment）,half of rats were killed at 4 weeks and 8 weeks all three group,respectively,detected the blood glucose,heart function,HO-1 and MMP-9 expression in heart tizzies. Results：Compared with the normal control group,the model group in blood glucose,heart function index of CK,CK-MB,LDH and HBDH significantly increased（P〈0.05）,after 4 weeks of treatment,all the above indices were significantly decreased（P〈0.05）,and after 8 weeks of treatment further reduced. HO-1 and MMP-9 immunohistochemistry,Western blot and RT-PCR can been seen the normal group compared model group,HO-1 expression was significantly decreased（P〈0.05）,MMP-9 was significantly increased（P〈0.05）;after treatment the HO-1 expression increased significantly（P〈0.05）,MMP-9 decreased significantly（P〈0.05）. Conclusion：Total saponins of bitter gourd had obvious effects on MMP-9 and HO-1 in heart of rat.

关 键 词：苦瓜总皂苷 2型糖尿病 心肌损伤 血红素加氧酶1 基质金属蛋白酶-9 

分 类 号：R589.9[医药卫生—内分泌]