多囊卵巢综合征患者颗粒细胞microRNA-200b的表达及影响  

Expression of microRNA-200b in granulosa cells of PCOS patients and its significance

作　　者：贾月月 刘洪彬[1] 李婧博 李敬[1] 张江涛[1] 孙梅[1] 石玉华[1] JIA Yueyue LIU Hongbin LI Jingbo LI Jing ZHANG Jiangtao SUN Mei SHI Yuhua(Center for Reproductive Medicine, Shandong University, National Research Center for Assisted Reproductive Technology and Reproductive Genetics, Key Laboratory for Reproductive Endocrinology （Shandong University）, Ministry of Education, Shandong Provincial Key Laboratory of Reproductive Medicine, Jinan 250001, Shandong, China)

机构地区：[1]山东大学附属生殖医院国家辅助生殖与优生工程技术研究中心生殖内分泌教育部重点实验室(山东大学)山东省生殖医学重点实验室,山东济南250001

出　　处：《山东大学学报（医学版）》2017年第1期63-68,共6页Journal of Shandong University:Health Sciences

基　　金：国家科技支撑计划(2012BAI32B04);国家自然基金面上项目(81471428;81200423);山东省科技发展计划(2014GSF118070);山东省人口和计划生育委员会科技计划项目(2013-03);山东大学青年学者未来计划(第二批;2016WLJH50);山东大学附属生殖医院自主创新项目(20141102;20141103)

摘　　要：目的检测多囊卵巢综合征(PCOS)患者卵巢颗粒细胞中microRNA-200b(miR-200b)的表达,分析其与胰岛素抵抗的关系。方法选取PCOS患者120例(其中胰岛素抵抗78例,非胰岛素抵抗42例)及对照患者72例。采用逆转录定量PCR(qRT-PCR)检测在卵巢颗粒细胞中miR-200b的表达,并分析其临床意义。结果 PCOS胰岛素抵抗患者中超重或肥胖、高血压、高血糖、脂代谢紊乱和代谢综合征的发生率皆高于PCOS非胰岛素抵抗患者和对照患者(P均<0.05)。PCOS胰岛素抵抗患者miR-200b表达上调,与PCOS非胰岛素抵抗患者和对照患者比较差异有统计学意义(P=0.012,P<0.001);且PCOS胰岛素抵抗患者中miR-200b表达量与胰岛素抵抗指数(HOMA-IR)呈正相关(r=0.532,P=0.002)。结论 PCOS胰岛素抵抗患者高血压等代谢方面远期并发症的患病率较高。miR-200b高表达与PCOS胰岛素抵抗呈正相关,可能是PCOS患者出现胰岛素抵抗的重要病理机制之一。Objective To explore the expression and clinicopathological significance of microRNA-200b（ miR-200b）in ovarian granulosa cells of patients suffering from polycystic ovary syndrome（ PCOS） with insulin resistance.Methods A total of 120 PCOS patients（ 78 with insulin resistance and 42 without） and 72 controls were enrolled. The expression of miR-200 b was examined in ovarian granulosa cells by real-time quantitative reverse transcription PCR（ qRT-PCR）,and the clinical significance was analyzed. Results PCOS patients with insulin resistance had higher morbidity of overweight,obesity,hypertensive disease,hyperglycemia,dyslipidemia and metabolic syndrome than PCOS patients without insulin resistance and controls（ all P〈0. 05）. Expression of miR-200 b was much higher in PCOS patients with insulin resistance than in PCOS patients without insulin resistance and controls（ P = 0. 012,P〈0. 001）. In PCOSpatients with insulin resistance,the index of homeostatic model assessment of insulin resistance（ HOMA-IR） was positively correlated with miR-200 b expression（ r = 0. 532,P = 0. 002）. Conclusion PCOS patients with insulin resistance have high morbidity of long-term metabolic complications. The high expression of miR-200 b is positively related to insulin resistance,which may be the underlying pathogenic mechanism for insulin resistance in PCOS patients.

关 键 词：microRNA-200b 多囊卵巢综合征 胰岛素抵抗 颗粒细胞 

分 类 号：R711.75[医药卫生—妇产科学]