microRNA-183在PCOS胰岛素抵抗中的表达及其临床意义  

Expression and clinical significance of microRNA-183 in polycystic ovary syndrome with insulin resistance

作　　者：李婧博 刘洪彬[1] 贾月月 王泽[1] 孙梅[1] 石玉华[1] LI Jingbo LIU Hongbin JIA Yueyue WANG Ze SUN Mei SHI Yuhua(Center for Reproductive Medicine, Shandong University, National Research Center for Assisted Reproductive Technology and Reproductive Genetics, Key Laboratory for Reproductive Endocrinology （Shandong University）, Ministry of Education,Shandong Provincial Key Laboratory of Reproductive Medicine, Jinan 250001, Shandong, China)

机构地区：[1]山东大学附属生殖医院国家辅助生殖与优生工程技术研究中心生殖内分泌教育部重点实验室(山东大学)山东省生殖医学重点实验室,山东济南250001

出　　处：《山东大学学报（医学版）》2017年第1期69-74,共6页Journal of Shandong University:Health Sciences

基　　金：国家科技支撑计划(2012BAI32B04);国家自然科学基金面上项目(81471428;81200423);山东省科技发展计划(2014GSF118070);山东省人口和计划生育委员会科技计划(2013-03);山东大学青年学者未来计划(第二批;2016WLJH50);山东大学附属生殖医院自主创新项目(20141102;20141103)

摘　　要：目的检测多囊卵巢综合征(PCOS)胰岛素抵抗患者卵巢颗粒细胞中microRNA-183(miR-183)的表达,并评价其临床意义。方法采用实时荧光定量逆转录聚合酶链反应(qRT-PCR)检测120例PCOS患者[其中78例PCOS胰岛素抵抗患者(PCOS胰岛素抵抗组)和42例PCOS非胰岛素抵抗患者(PCOS非胰岛素抵抗组)及72例正常对照者(正常对照组)]颗粒细胞中miR-183的表达,并对miR-183表达和胰岛素抵抗指数(HOMA-IR)进行关联分析,同时对组间临床资料进行回顾性分析。采用受试者工作特征(ROC)曲线评价miR-183的诊断效能。结果 miR-183在PCOS胰岛素抵抗组颗粒细胞中的表达高于正常对照组(P<0.001)及非胰岛素抵抗组(P=0.034),PCOS胰岛素抵抗组miR-183的表达与HOM A-IR呈正相关(r=0.476,P=0.003),ROC曲线提示miR-183联合体质量指数(BM I)作为PCOS胰岛素抵抗的评价指标,其诊断效能较高(AUC:0.820,95%CI:0.750~0.889,P<0.001)。结论 miR-183在PCOS胰岛素抵抗患者颗粒细胞中高表达,并与HOM A-IR相关,其可能通过卵泡局部微环境参与调控PCOS胰岛素抵抗的致病过程。Objective To explore the expression of microRNA-183（ miR-183） in the ovarian granulosa cells of polycystic ovary syndrome（ PCOS） patients with insulin resistance（ IR）,and to evaluate its clinical significance. Methods A total of 120 PCOS patients including 78 with insulin resistance and 42 without,and 72 healthy controls were enrolled. The miR-183 expression was examined by real-time quantitative reverse transcription PCR（ qRT-PCR）. The correlation between miR-183 and homeostasis model assessment-insulin resistance（ HOMA-IR） was analyzed. The area under the receiver operating characteristic（ ROC） curve（ AUC） was calculated to evaluate the predictive power of miR-183 for PCOS insulin resistance. Results PCOS patients with insulin resistance had higher miR-183 expression than the control group（ P 0. 001） and PCOS without insulin resistance group（ P = 0. 034）. There was positive correlationbetween HOMA-IR and miR-183 expression in PCOS with insulin resistance group（ r = 0. 476,P = 0. 003）. The ROC analysis suggested that combination of miR-183 and body mass index（ BMI）（ AUC： 0. 820,95% CI： 0. 750-0. 889,P 0. 001） might be an effective indicator. Conclusion The high expression of miR-183 is correlated to insulin resistance of PCOS and HOMA-IR,and may be involved in the pathogenesis of PCOS insulin resistance through follicular microenvironment.

关 键 词：miR-183 多囊卵巢综合征 胰岛素抵抗 颗粒细胞 

分 类 号：R711.75[医药卫生—妇产科学]