依达拉奉对百草枯致大鼠肾脏氧化应激因子及HO-1表达的影响  

Edaravone Adjustes the Expression of the Oxidative Stress Factor and HO-1 Expression in Renal of Paraquat Poisoning Rat

作　　者：蔡旗旗[1] 张盛[1] 应盼 葛小刚[2] 任益群[1] 吴晓玉[1] 杨志辉[1] 

机构地区：[1]台州市第一人民医院EICU,318020 [2]台州市第一人民医院外科,318020

出　　处：《医学研究杂志》2017年第1期101-104,共4页Journal of Medical Research

基　　金：台州市科技局基金资助项目(131KY15)

摘　　要：目的观察依达拉奉(Eda)对百草枯(PQ)急性中毒所致大鼠肾脏氧化应激因子、HO-1蛋白表达的影响。方法通过腹腔注射百草枯建立百草枯中毒模型,并予以依达拉奉腹腔注射进行干预,ELISA法观察氧化应激因子的表达情况。Western blot法监测HO-1蛋白表达情况。结果 PQ组在1、3及7天时CAT、SOD水平较低,21天时明显升高,MDA在1天时表达水平较高,随后逐渐下降,21天时有所回升(P<0.05);PQ+Eda组1天及3天时CAT、SOD及MDA表达水平均较NS组低,7天时均有所升高,SOD表达呈现峰值,21天时表达接近NS组(P<0.05)。Eda组CAT及MDA表达较NS组差异无统计学意义(P>0.05),而SOD表达随时间延长逐渐升高。肾脏组织中HO-1表达Eda组及PQ+Eda组较PQ组显著性增加(P<0.05)。结论依达拉奉可促使肾脏组织SOD及CAT表达提高,MDA表达降低,并可上调HO-1表达,对肾脏损伤起到保护作用。Objective To observe the expression of oxidative stress factor and HO - I in renal adjusted by Edaravone of paraquat poisoning rat. Methods Edaravone was injected after paraquat poisoning model setting up. Then we observed the HO - 1 protein expres- sion by Western blot in 1,3,7,21d. We observed CAT, MDA, SOD protein expression by ELISA. Results In PQ group, the level of CAT, SOD were low in 1,3 and 7d, then significantly increased in 21d, MDA expression was higher in 1 d, then gradually decline, recov- ered in 21d （P 〈0.05）. In PQ ＋ Eda group, the expression level of CAT, SOD and MDA was lower than those of NS group in ld and 3d, and all was increased in 7d, and SOD expression present peak （P 〈 0.05 ）. In Eda group,the expression level of CAT and MDA had no obvious difference in the NS group, and the SOD expression extended increased over time. Expression of HO - 1 was significantly in- creased in PQ ＋ EDA group,and it was significantly increased than the PQ group （P 〈 0.05 ）. Conclusion Edaracone can promote the expression of SOD and CAT in kidney tissue, decrease MDA expression, and raise HO - 1 expression. Eventually it can protect the renal function.

关 键 词：百草枯 氧化应激 肾损伤 HO-1 HO-1 

分 类 号：R3[医药卫生—基础医学] R4