活动期肺结核外周血mir-144的表达水平及意义  

Expression Level and Clinical Significance of mir-144 in Peripheral Blood of Active Pulmonary Tuberculosis Patients

作　　者：张立营[1] 陈朴[1] 高鹏[1] 沈伟[1] 

机构地区：[1]宜宾市第一人民医院检验科,644000

出　　处：《医学研究杂志》2017年第1期151-154,共4页Journal of Medical Research

摘　　要：目的探讨活动期肺结核患者外周血mir-144表达水平及临床意义。方法选择2014年1月~2015年1月在笔者医院就诊的20例活动期肺结核患者及20例健康体检者作为研究对象,收集其静脉血,分别提取两组患者外周血单个核细胞中总RNA,采用实时荧光定量PCR比较其外周血mir-144的表达水平情况,采用受试者工作特征曲线(receiver operating characteristic curve,ROC)评估mir-144的诊断效能。同时采用ELISA法测定两组研究对象血浆中的IL-6、TNF-α、IFN-γ的水平。进一步进行相关性分析评估外周血mir-144表达水平与IL-6、TNF-α、IFN-γ的相关性。结果活动期肺结核患者外周血中mir-144表达水平相比较健康对照组高,差异有统计学意义(P>0.05)。活动期肺结核患者外周血中IL-6、TNF-α、IFN-γ的水平比健康对照组要高,差异有统计学意义(P<0.05)。ROC下面积为0.885,当最佳筛查阳性界值(cut-off值)为0.955时,敏感度和特异性均为85%。活动期肺结核外周血中mir-144的表达水平与其血浆中的IL-6、TNF-α、IFN-γ的水平呈负相关,相关系数分别为-0.905、-0.910、-0.892。结论外周血单个核细胞中mir-144的表达水平可能作为活动期肺结核诊断的一个血清学指标,mir-144在结核杆菌感染后免疫反应中可能与外周血IL-6、TNF-α、IFN-γ的表达水平有关。Objective To evaluate the diagnostic values of microRNA - 144 in peripheral blood of active pulmonary tuberculosis pa, tients. Methods Twenty active pulmonary tuberculosis patients were selected between Jan 2014 and Jan 2015,and twenty healthy peo- ple, selected in parallel, served as the control group. Real - time PCR was used to detect mir - 144 expression in PBMCs. Receiver oper- ating characteristic curve （ROC） was employed to evaluate the diagnostic value of mir- 144 in active pulmonary tuberculosis. Serum lev- els of IL - 6, TNF - α and IFN - γ was detected by ELISA. Pearson correlation coefficient was used to evaluate the correlation between mir - 144 expression and serum levels of IL - 6, TNF -α and IFN - γ. Results The expression of mir - 144 in active pulmonary tuber- culosis patients was signifiantly higher than that in normal people. Serum levels of IL - 6, TNF - α and IFN - γ were significantly higher in pediatric TB patients compared with normal people. The area under the curve （AUC） was 0. 855. The cut - off threshold of mir - 144 for active pulmonary tuberculosis diagnosis was 0. 955 with a sensitivity of 85% and a specificity of 85%. Furthermore, mir- 144 expres- sion was negatively correlated with serum levels of IL - 6 （ r = - 0. 905 ）, TNF - ct （ r = - 0. 910）, and IFN -γ （ r = - 0. 892）. Conclusion mir - 144 has the potential to be a diagnostic marker in active pulmonary tuberculosis patients. Moreover,the role of mir - 144 in the immune response of mycobacterium tuberculosis infection may be related with serum levels of IL- 6, TNF-α, IFN- γ.

关 键 词：结核 外周血 Mir-144 细胞因子 Mir-144 

分 类 号：R446[医药卫生—诊断学]