芪冬活血饮对LPS致炎巨噬细胞炎症反应的作用及机制探讨  

Effects and Mechanism of Qidong Huoxue Decoction on Inflammation of Macrophages Stimulated by LPS

作　　者：洪辉华[1] 赵玮[1] 朱渊红[1] 蔡宛如[2] 

机构地区：[1]浙江中医药大学附属第一医院呼吸科,浙江杭州310006 [2]浙江中医药大学附属第二医院呼吸科,浙江杭州310005

出　　处：《中华中医药学刊》2017年第1期47-50,共4页Chinese Archives of Traditional Chinese Medicine

基　　金：国家自然科学基金项目(81273678;81603571);浙江省自然科学基金项目(LQ12H29003)

摘　　要：目的:通过观察芪冬活血饮大鼠药物血清对NF-κB抑制巨噬细胞炎症细胞因子及TLR4、Cav-1、NF-κBp65 mRNA表达的影响,探讨其炎症调控的机制。方法:将巨噬细胞分为空白对照组(UT组)、LPS组、NF-κB抑制组(NI组)、NF-κB抑制+LPS组(NL组)、LPS加空白血清组(LB组)、LPS加药物血清组(LD组)、NF-κB抑制+LPS+空白血清组(NLB组),NF-κB抑制+LPS+药物血清组(NLD组)8组。巨噬细胞予阻断剂阻断0.5 h后加入LPS致炎,并予药物血清干预,LPS处理12 h后测定各组细胞上清液TNF-α、IL-1β、IL-10水平,及TLR4、Cav-1、NF-κBp65 mRNA相对表达量。结果:LD组TNF-α、IL-1β、IL-10水平及NF-κBp65、Cav-1、TLR4 mRNA相对表达量均低于LB组和LPS组,比较有统计学意义;NLD组TNF-α、IL-1β、IL-10水平及NF-κBp65、Cav-1、TLR4mRNA相对表达量均低于NL组,两组TNF-α、IL-1β有显著统计学差异,NF-κBp65mRNA、IL-10比较差异无统计学意义,Cav-1、TLR4mRNA比较差异有统计学意义。结论:芪冬活血饮药物血清可减轻LPS刺激的巨噬细胞炎性反应;除TLR4/Cav-1/NF-κBp65途径外,芪冬活血饮还可以通过非NF-κBp65途径降低炎症反应。Objectives ： To observe the effects of Qidong Huoxue Decoction （QD） drug - serum on inflammation of NF - KB -inhibited macrophages stimulated by LPS and explore the mechanism. Methods：The macrophages were divided into eight groups ： untreated group （ UT）, LPS group, NF - KB - inhibited group （ NI）, NF -κB - inhibited plus LPS group （NL）, LPS plus blank serum group（ LB）, LPS plus drug serum group（ LD）, NF -κB -inhibited plus LPS and blank ser- um group（ NLB）, NF- κB -inhibited plus LPS and drug serum group（NLD）. NF-κB was inhibited by SN50 and cul- tured for 0.5 hour and then LPS was added and cultured for another 12 hours. Levels of TNF -a,IL -1β and IL- 10 in cell supernatant, mRNA relative expressions of TLR4, Cav - 1 and NF - KBp65 of each group were detected. Results ： The levels of TNF- α, IL- 1β,1L- 10 and mRNA relative expressions of TLR4, Cav- 1 and NF- κBp65 of ND group were lower than those of LPS and LB groups and there were statistical differences. The levels of TNF -α, IL - 1β, IL - i0 and mRNA relative expressions of TLR4, Cav - 1 and NF - κBp65 of NLD group were all lower than the NL group＇ s. The comparison of TNF -α and IL - 1β of two groups had significant statistical differences but there was no statistical difference between NF -κBp65mRNA and IL - 10 while comparison of Cav - 1 and TLR4 had statistical differences. Conclusions：QD drug - serum could relieve the inflammatory response of macrophages stimulated by LPS. It could inhibit TLR4/Cav - 1/NF - κBp65 signaling pathways and NF - κBp65 - indepent signaling pathways.

关 键 词：芪冬活血饮 急性肺损伤 TOLL样受体4 小窝蛋白-1 核转录因子KB 细胞因子 

分 类 号：R285.5[医药卫生—中药学]