冬虫夏草提取物对结肠癌HT-29细胞增殖和凋亡的影响及其机制  

Effect of Cordyceps sinensis Extracts on Proliferation and Apoptosis of Human Colon Cancer HT-29 Cells and Its Mechanism

作　　者：牛明了[1] 甄欢欢[1] 周晓丽[2] 席作武[1] 

机构地区：[1]河南省中医院,河南郑州450002 [2]河南中医学院第三附属医院,河南郑州450008

出　　处：《中华中医药学刊》2017年第1期73-75,共3页Chinese Archives of Traditional Chinese Medicine

基　　金：国家中医药管理局全国名老中医传承工作室项目(201347)

摘　　要：目的:探讨冬虫夏草提取物对结肠癌细胞株HT-29细胞增殖、凋亡的影响及其机制。方法:利用不同浓度冬虫夏草提取物(0.5~6.5 mg/m L)作用HT-29细胞24、48和72 h,采用MTT、平板实验、流式细胞检测和WB检测冬虫夏草提取物对HT-29细胞生长增殖作用、细胞集落数的形成、对细胞凋亡率的影响以及对细胞凋亡相关分子Caspase-3蛋白表达的影响。结果:MTT结果表明,提取物能够显著抑制HT-29的生长增殖,呈一定的量时效关系,半数抑制浓度为3.5 mg/m L;流式细胞仪分析表明,冬虫夏草提取物呈浓度依赖性诱导HT-29细胞凋亡,浓度为6.5 mg/m L时,HT-29凋亡率(12.57±0.16)%;Western blotting表明,HT-29细胞与对照组相比,经不同浓度提取物诱导后,Caspase-3表达逐渐增强。结论:冬虫夏草提取物具有抑制HT-29细胞增殖作用,这可能与增加Caspase-3蛋白表达、诱导细胞调亡有关。Objective： To investigate the effect of Cordyceps sinensis extracts on the proliferation and apoptosis of HT - 29 cells and the relevant molecular mechanism. Methods： The anti - proliferative effect of different concentrations of Cordyceps sinensis on HT - 29 was measured by MTT assay. Colony - forming capacity was measured by trypan blue staining. The apoptosis ratio of HT - 29 was analyzed by flow cytometry. The expression level of Casppase - 3 was analyzed by Western blot. Results ：The MTT assay showed that Cordyceps sinensis had potent anti -proliferative effect on HT- 29 ceils in time and dose dependent manner. ICs0 was 3.5 mg/mL. Flow cytometry analysis revealed that Cordyceps sinensis increased the percentage of apoptotic HT- 29 cells and the apoptosis rate was （12.5 ＋ 0.16）% when the concentration was 6.5 mg/mL. The expression level of Caspase - 3 was increased significantly in HT - 29 cells treated with different concentrations of Cordyceps sinensis. Conclusion ： Cordyceps sinensis inhibits the proliferation and induces apoptosis of HT -29 cells by increasing Caspase- 3 expression.

关 键 词：冬虫夏草 结肠癌 增殖 凋亡 

分 类 号：R285.5[医药卫生—中药学]