“FQ-PCR溶解曲线法”监测麻风患者TCRa链CDR3谱系漂移的研究  

Study on the FQ—PCR Dissolution Curve Method for Monitoring a TCR Chain CDR3 in Leprosy Patients

作　　者：张力丹 张庆波 杨小静 韦蓉 陈利远 姚新生[2] 

机构地区：[1]贵阳市第二人民医院皮肤科,贵州贵阳550001 [2]遵义医学院基础医学院免疫学教研室,贵州遵义563000

出　　处：《中国继续医学教育》2016年第35期45-46,共2页China Continuing Medical Education

摘　　要：麻风病是由麻风杆菌引起的一种慢性接触性传染病。主要侵犯人体皮肤和神经,如果不治疗可引起皮肤、神经等永久性的损害。当下,我国对麻风病的治疗以预防为主,而对于那些已经患上麻风病的患者来说,治疗方法还值得更深层次的研究探索。本篇文章的研究方法是通过T细胞受体a链V区基因各家族CDR3免疫谱系分析技术来监测麻风患者TCR a链CDR3谱系的漂移情况,进而初步分析出TRAV家族的CDR3 PCR产物定量和克隆增生情况,以此为被麻风感染后的患者的T细胞免疫应答变化提供新的研究方向。Leprosy is a chronic infectious diseasecaused by Mycobacterium leprae. Majorviolations of human skin and nerves, if not treatment can cause permanent damage to the skin, nerves, etc. At present, Chinese treatment of leprosy prevention, and for those patients who have been suffering from leprosy, treatment is also worth studying deeper exploration. The research method of this article is through the T cellreceptor gene drift a chain V region each family CDR3 immune pedigree analysis technique to monitor leprosy patient TCR a chain CDR3 pedigree, and preliminary analysis of CDR3 PCR products and TRAVcloning of quantitative hyperplasia of the family, in order to provide a new research direction for T cell immune response changes after the patients infected with leprosy.

关 键 词：CDR3 FQ-PCR 溶解曲线 

分 类 号：R440[医药卫生—诊断学]