鸡减蛋综合征灭活抗原不同保存方式的分析比较  

Analysis and Comparison of Different Preservation Methods of Inactivated Antigen of Chicken Egg Drop Syndrome

作　　者：王嘉[1] 顾庆云[2] 金红岩[2] 毛娅卿[1] 吴涛[1] 王哲[1] 孔冬妮[1] 李岭[1] WANG Jia GU Qing -yUN JIN Hong -yan MAO Ya -qing WU Tao WANG Zhe KONG Dong -ni LI Ling(China Institute of Veterinary Drug Control, Beijing 100081, China Tibet Vocational Technical College, Lhasa 850030, China)

机构地区：[1]中国兽医药品监察所,北京100081 [2]西藏职业技术学院,拉萨850030

出　　处：《中国兽药杂志》2017年第1期12-14,共3页Chinese Journal of Veterinary Drug

摘　　要：为比较不同保存方式对鸡减蛋综合征灭活抗原的影响,采用禽腺病毒京911株(CVCC AV70)经尿囊腔接种易感鸭胚,收获感染鸭胚液,经β-丙内酯灭活后,对该抗原的保存条件及其稳定性等方面进行优化。冻干抗原与未冻干抗原在相同温度下,冻干抗原HA效价下降较慢。冻干抗原在2~8℃和-15℃以下保存,HA效价没有明显差异。因此,生产的鸡减蛋综合征灭活抗原要在冻干后保存在2~8℃最佳。保存条件的优化不仅可以减少经济损失,而且还有助于更好的控制禽腺病毒疫情的暴发。In order to compare the effects of different preservation methods on the inactivated antigen of chicken egg drop syndrome, avian adenovirus Beijing 911 strain （CVCC AV70） was inoculated in duck embryo allantoic cavity. Optimizing the preservation conditions and stability of antigen after the harvested virus was inactivated by β propiolactone. Comparison of the freeze dried antigen and the non freeze dried antigen at the same temperature, the titer of freeze - dried antigen HA decreased slowly, and there was no significant difference in HA titer at different temperatures. Therefore, the freeze dried and inactivated antigen of the chicken egg drop syndrome should be kept at 2 - 8℃. Optimization of preservation conditions can not only reduce economic losses, but also contribute to a better control of the outbreak of avian adenovirus.

关 键 词：鸡减蛋综合征 禽腺病毒 HA效价 保存方式 

分 类 号：S858.31[农业科学—临床兽医学]