溃结灵对溃疡性结肠炎大鼠肠道Treg相关因子的调控作用  

Regulatory Effects of Kuijieling on Treg-related Factors of Experimental Ulcerative Colitis Rats

作　　者：甘丽萍[1] 王凤[1] 龙宇[1] 李燕舞[1] 巫燕莉[1] 杜群[1] GAN Liping WANG Feng LONG Yu LI Yanwu WU Yanli DU Qun(Pi-Wei Institute, Guangzhou University of Chinese Medicine, Guangzhou 510405 Guangdong, China)

机构地区：[1]广州中医药大学脾胃研究所,广东广州510405

出　　处：《中药新药与临床药理》2017年第1期13-18,共6页Traditional Chinese Drug Research and Clinical Pharmacology

基　　金：国家自然科学基金(81373798);广州中医药大学中医药防治脾胃病;脑病创新研究团队项目(2016KYTD07)

摘　　要：目的观察溃结灵对溃疡性结肠炎(UC)大鼠模型结肠黏膜调节性T细胞(Treg)相关因子,Foxp3、STAT5的调控作用,探讨溃结灵防治UC的作用机理。方法采用三硝基苯磺酸(TNBS)法复制UC大鼠模型并进行中药复方溃结灵药物干预治疗。采用酶联免疫吸附法(ELISA)检测结肠黏膜白细胞介素2(IL-2)、白细胞介素10(IL-10)的表达,免疫组化方法检测结肠黏膜蛋白原位表达,并采用实时荧光定量PCR(RT-PCR)方法检测Foxp3、STAT5基因表达。结果模型组结肠黏膜IL-2、IL-10表达量均低于正常组(P<0.05,P<0.01),溃结灵高剂量组及阳性药物组柳氮磺胺吡啶(SASP),IL-2表达量高于模型组(P<0.01),溃结灵高、中剂量组及阳性药物组IL-10表达量均高于模型组(P<0.05,P<0.01);免疫组化检测模型组结肠黏膜Foxp3、STAT5的原位蛋白表达低于正常组(P<0.01),溃结灵高、中、低剂量组及阳性药物组Foxp3、STAT5的原位蛋白表达均高于模型组(P<0.05,P<0.01);模型组Foxp3、STAT5 m RNA表达量低于正常组(P<0.05,P<0.01),而溃结灵高、中剂量组及阳性药物组Foxp3 m RNA表达高于模型组(P<0.05,P<0.01),溃结灵高剂量组及阳性药物组STAT5 m RNA高于模型组(P<0.05,P<0.01)。结论溃结灵对UC大鼠模型结肠黏膜IL-2、IL-10含量以及Foxp3、STAT5原位蛋白和基因表达的上调作用,可能是其促进Treg细胞的分化,发挥治疗UC作用的机制之一。Objective To observe the regulatory effect of Kuijieling Decoction（KD） on Treg-regulated factors of interleukin-2（IL-2）,interleukin-10（IL-10）,fork head box p3（Foxp3） and signal transduction and activator of transcription 5（STAT5）in colonic mucosa of rats with ulcerative colitis（UC）,and to explore its possible mechanism.Methods Trinitro-benzene-sulfonic acid（TNBS）was used to establish UC rat model. After the rats were treated with different dosages of KD,the contents of IL-2 and IL-10 in rats were determined using enzyme-linked immunosorbent assay（ELISA）kit,and immunohistochemical technique was used to detect the expression levels of Foxp3 and STAT5 in colonic mucosa. Real-time guantitative PCR was used to measure m RNA expression of Foxp3 and STAT5. Results The contents of IL-2 and IL-10 in colonic mucosa of model group were lower than those of the normal group（P〈0.05,P〈0.01）,and the content of IL-2 in high-dosage KD group and positive drug salazosulfapyridine（SASP）group was significantly higher than that in the model group（P〈0.01）. The content of IL-10 in colonic mucosa of high- and middle-dosage groups and in SASP group was also significantly higher than that in the model group（P〈0.05,P〈0.01）. The in-situ protein expression of Foxp3 and STAT5 in model group was notably lower than that in normal group（P〈0.01）,and the expression of Foxp3 and STAT5 in high-,middle-,and low-dosage KD groups and SASP group was obviously higher than that the in the model group（P〈0.05,P〈0.01）. The m RNA expression of Foxp3 and STAT5 in the model group was notably lower than that in the normal group（P〈0.05,P〈0.01）,while the Foxp3 m RNA expression in high- and middle-dosage KD groups and SASP group was obviously higher than that in the model group（P〈0.05,P〈0.01）. The STAT5 m RNA expression in high-dosage KD group and SASP group was obviously higher than that in the model group（P〈0.05,P〈0.01）. Conclusion KD can up-regulate the relative express

关 键 词：溃结灵 溃疡性结肠炎 IL-2 IL-10 FOXP3 STAT5 调节性T细胞 

分 类 号：R285.5[医药卫生—中药学]