黄芪-葛根药对保护吲哚美辛诱导的大鼠胃黏膜损伤的作用研究  

Effect of Drug Pair Huangqi-Gegen on Protecting Rat Gastric Mucosa from Injury Induced by Indomethacin

作　　者：林静瑜[1,2] 黄可儿[3] 秦书敏[2] 周凡[1] 程佑民[1] LIN Jingyu HUANG Keer QIN Shumin ZHOU Fan CHENG Youmin(Fujian Institute of Traditional Chinese Medicine, Fuzhou 350003 Fujian, China Guangzhou University of Chinese Medicine, Guangzhou 510405 Guangdong, China The First Affiliated Hospital of Guangzhou University of Chinese Medicine, Guangzhou 510405 Guangdong, China)

机构地区：[1]福建省中医药研究院,福建福州350003 [2]广州中医药大学,广东广州510405 [3]广州中医药大学第一附属医院,广东广州510405

出　　处：《中药新药与临床药理》2017年第1期32-35,共4页Traditional Chinese Drug Research and Clinical Pharmacology

基　　金：广东省省级科技计划项目(20138090600020);福建省属公益类科研院所专项(2016R1033-1)

摘　　要：目的探讨黄芪-葛根药对对吲哚美辛诱导的大鼠胃黏膜损伤的保护作用。方法取SD大鼠,按体质量分层随机分为正常组,模型组,阳性药组(西咪替丁0.12 g·kg^(-1)),药对高、中、低剂量组(黄芪-葛根药对水煎液24,12,6 g·kg^(-1)),连续灌胃4 d,第4天给药后75 min,除正常组外,其余各组均腹腔注射吲哚美辛混悬液35 mg·kg^(-1)造模,观察胃黏膜损伤分数、胃黏膜病理学改变,检测胃黏膜组织丙二醛(MDA)、超氧化物歧化酶(SOD)、活性氧自由基(ROS)的水平。结果模型组大鼠胃黏膜出血、糜烂、损伤明显,胃黏膜组织ROS、MDA含量上升,SOD活力下降。与模型组比较,黄芪-葛根药对各剂量组能不同程度地改善大鼠胃黏膜外观及病理学的变化,降低胃黏膜损伤分数(P<0.05,P<0.01);同时,明显减少大鼠胃黏膜组织MDA、ROS含量(P<0.05,P<0.01);黄芪-葛根药对高剂量组能明显提高SOD活力(P<0.05)。结论黄芪-葛根药对对吲哚美辛诱导的大鼠胃黏膜损伤有一定保护作用,其作用可能与抗胃黏膜组织氧化应激水平有关。Objective To explore the protective effect of drug pair Huangqi-Gegen（Radix Astragali-Radix Puerariae）on indomethacin-induced gastric mucosa damage in rats. Methods SD rats were randomly divided into normal group,model group,positive drug group（cimetidine 12 mg·kg-1·d-1,ig）,and high-,medium- and low-dose HuangqiGegen water decoction group（24, 12, 6 g·kg-1·d-1, ig） according to the body weight of rats. The intragastric administration for each group lasted 4 continuous days. Seventy-five minutes after the last medication,all rats except for the normal group were intraperitoneally injected with indomethacin（35 mg·kg-1）to induce gastric mucosa injury. Six hours after indomethacin treatment,the animals were executed for the sampling of the stomach. The mucosal injury was scored, and the pathological changes in gastric mucosa were observed. The contents of malondialdehyde（MDA）,superoxide dismutase（SOD）,and reactive oxygen species（ROS）in mucosal tissue were detected. Results The gastric mucosal injury of model group was severer than the normal group,showing as obvious gastric mucosal lesions and erosion,increased ROS and MDA contents,and decreased SOD activity. The drug pair groups at different dosages had an effect on relieving the mucosal injury and pathological changes,and decreased gastric mucosal injury scores as well as the MDA and ROS contents（P〈0.05,P〈0.01 compared with those in the model group）. The high-dose drug pair enhanced SOD activity（P〈0.05 compared with that in the model group）. Conclusion Drug pair Huangqi-Gegen exerts certain protective effect on indomethacin-induced gastric mucosa injury,and the mechanism may be related to the counteraction of oxidative stress.

关 键 词：黄芪-葛根药对 吲哚美辛 胃黏膜损伤 氧化应激 

分 类 号：R285.5[医药卫生—中药学]