UFLC法同时测定龙胆中3种环烯醚萜苷的含量  

Simultaneous Determination of Three Iridoid Glycosides in Radix Gentianae by UFLC

作　　者：关皎[1] 朱鹤云[1] 郝乘仪[1] 郭淑英[1] 冯波[1] GUAN Jiao ZHU Heyun HAO Chengyi GUO Shuying FENG Bo(College of Pharmacy, Jilin Medical University, Changchun 132013 Jilin, China)

机构地区：[1]吉林医药学院药学院,吉林吉林132013

出　　处：《中药新药与临床药理》2017年第1期81-84,共4页Traditional Chinese Drug Research and Clinical Pharmacology

基　　金：吉林省卫生厅科研课题(2013Z008)

摘　　要：目的采用超快速液相色谱(UFLC)法同时测定龙胆中龙胆苦苷、獐芽菜苦苷和獐芽菜苷的含量。方法采用Shim-Pack XR-ODS柱(75 mm×3.0 mm,2.2μm),流动相为甲醇-0.1%磷酸水溶液(梯度洗脱),检测波长为240 nm,流速为0.5 m L·min^(-1),柱温为40℃。结果龙胆苦苷、獐芽菜苦苷和獐芽菜苷进样量分别在1.0~20μg(r=0.9998)、1.0~20μg(r=0.9995)、0.12~2.4μg(r=0.9996)范围内与峰面积呈良好的线性关系,平均加样回收率分别为97.3%、99.3%和98.3%(n=3),RSD均小于1.0%。结论 UFLC法简便、快速、准确、重现性好,可用于龙胆药材中环烯醚萜苷类成分的质量控制研究。Objective To develop an ultra-fast liquid chromatography（UFLC）method for simultaneous determination of gentiopicroside,swertiamarin and sweroside in Radix Gentianae. Methods Chromatographic separation was carried out on a Shim-Pack XR-ODS column（75 mm×3.0 mm,2.2 μm）with a mobile phase consisting of acetonitrile and water（containing 0.1 % phosphoric acid）by gradient elution at a flow rate of 0.5 m L·min-1. The detecting wavelength was set at 240 nm and the column temperature was maintained at 40 ℃. Results The calibration curves of gentiopicroside,swertiamarin and sweroside showed good linear relationship in the range of 1.0-20 μg（r=0.9998）,1.0-20 μg（r=0.9995）and 0.12-2.4 μg（r=0.9996）respectively,and the average recovery（n=9）of three components was 97.3 %, 99.3 % and 98.3 % respectively, and the relative standard deviation（RSD） was less than 1.0 %.Conclusion The developed UFLC method is simple, rapid, accurate and repeatable, which is suitable for the quality control of gentiopicroside,swertiamarin and sweroside in Radix Gentianae.

关 键 词：龙胆 龙胆苦苷 獐芽菜苦苷 獐芽菜苷 超快速液相色谱法 

分 类 号：R284.1[医药卫生—中药学]