银屑病患者皮损DMSCs血管生成相关基因mRNA的表达  

Angiogenesis-related m RNA Expressions in Dermal Mesenchymal Stromal Cells(DMSCs) from Patients with Psoriasis Vulgaris

作　　者：赵新程[1] 王强[1] 梁见楠 李娟[1] 杨晓红[1] 周玲[1] 李俊琴[1] 刘瑞风[1] 张开明[1] ZHAO Xin-cheng WANG Qiang LIANG Jian-nan LI Juan YANG Xiao-hong ZHOU Ling LI Jun-qin LIU Rui-feng ZHANG Kai-ming(Department of Dermatology, Taiyuan Central Hospital, Taiyuan 030009, Chin)

机构地区：[1]太原市中心医院皮肤科,免疫性皮肤病山西省重点实验室,山西太原030006

出　　处：《中国皮肤性病学杂志》2017年第2期140-143,共4页The Chinese Journal of Dermatovenereology

基　　金：国家自然科学基金面上项目(81472888)

摘　　要：目的检测寻常性银屑病患者皮损处和正常皮肤组织真皮间充质干细胞(DMSCs)中CXCL12,THBS1及THBS2的表达,探讨血管生成在银屑病发病中的作用。方法取15例寻常性银屑病患者皮损处和正常皮肤组织,分离、培养、扩增DMSCs,应用实时荧光定量PCR(RT-q PCR)检测DMSCs中CXCL12,THBS1及THBS2的表达。结果寻常性银屑病皮损处DMSCs表达CXCL12,THBS1及THBS2水平均明显高于正常对照组。结论银屑病患者皮损处血管生成相关基因CXCL12,THBS1及THBS2的m RNA表达增高。Objective In order to elucidate the role of angiogenesis in the pathogenesis of psoriasis, expressions of CXCL12,THBS1 and THBS2 in DMSCs from psoriatic lesions and healthy skin were investigated Methods There were 15 patients with psoriasis vulgaris and 15 healthy controls enrolled in this study. DMSCs from psoriatic lesions and healthy skin were isolated and purified. Real-time fluorescent quantitative polymerase chain reaction （RT-qPCR） was used to determine the expression of CXCL12, THBS1 and THBS2 in DMSCs. Results Expressions of CXCL12, THBS1, THBS2 in psoriatic lesions were remarkably higher than those in healthy controls. Conclusion Angiogenesis-related mRNA expressions, such as CXCL12, THBS1 and THBS2, were significantly higher in the psoriatic lesions.

关 键 词：银屑病 皮肤间充质干细胞 血管生成 CXCL12 THBS1 THBS2 

分 类 号：R758.63[医药卫生—皮肤病学与性病学]