HPLC法测定山黄胶囊中盐酸小檗碱和黄芩苷的含量  

Determination of berberine hydrochloride and baicalin in Shanhuang capsules by HPLC

作　　者：杨绪芳[1,2] 吴云[1,2] 韦迎春[1,2] 康小东[1,2] 李峰[1,2] 李明慧[1,2] 

机构地区：[1]江苏康缘药业股份有限公司,江苏连云港222001 [2]中药制药过程新技术国家重点实验室,江苏连云港222001

出　　处：《机电信息》2017年第2期31-35,共5页

摘　　要：目的:建立山黄胶囊中盐酸小檗碱、黄芩苷含量测定的方法。方法:采用HPLC法,测定盐酸小檗碱含量的色谱柱为Phenomenex Gemini C18柱(250 mm×4.6 mm,5μm),流动相为乙腈-0.1%磷酸(50:50),每100 m L加0.1 g十二烷基磺酸钠,流速为1.0 m L/min,检测波长为265 nm;测定黄芩苷含量的色谱柱为Phenomenex Luna C18柱(250 mm×4.6 mm,5μm),流动相为甲醇-水-磷酸(70:30:0.2),检测波长280 nm,流速0.6 m L/min。结果:盐酸小檗碱在5.08~50.80 g/m L范围内线性关系良好,R2=0.999 8,平均回收率为98.08%,RSD为1.09%。黄芩苷在15.03~150.30 g/m L范围内线性关系良好,R2=0.999 9,平均回收率为98.13%,RSD为1.28%。结论:该方法专属性强、准确性高、重复性好,可应用于山黄胶囊的质量控制。Objective To develop a method for the determination of berberine hydrochloride and baicalin in Shanhuang capsule. Methods The assay of berberine hydrochloride was performed on Phenomenex Gemini C18column(250 mm× 4.6 mm,5 μm)with a DAD 265 nm.The mobile phase was acetonitrile-0.01% phosphate(50:50),join 0.1 g sodium dodecyl sulfate per 100 m L solution with a flow rate of 1.0 m L/min. The determination of baicalin was on a Phenomenex Luna C18column(250 mm ×4.6 mm,5 μm)with a DAD 280 nm.The mobile phase was methanol-water – phosphate(70:30:0.2) with a flow rate of 0.6 m L/min. Results The linear range of berberine hydrochloride was 5.08 ~50.80 g/m L,R2=0.999 8,and the average recovery ratio was 98.08%,RSD=1.09%; The linear range of baicalin was 15.03~150.30 g/m L,R2=0.999 9,and the average recovery ratio was 98.13%,RSD=1.28%.Conclusion The method is exclusive,accurate and reproducible,which may be applied in the quality control of Shanhuang capsule.

关 键 词：山黄胶囊 HPLC法 盐酸小檗碱 黄芩苷 

分 类 号：R286.0[医药卫生—中药学] O657.72[医药卫生—中医学]