邱百灵,吴迪,郭爽,朱尘琪,高杨亚雅,梁启慧,高也,宋宇,韩南银＊  

Optimization of experimental conditions for the separation of polystyrene particles by gravitational field-flow fractionation

作　　者：邱百灵 吴迪 郭爽 朱尘琪 高杨亚雅 梁启慧 高也 宋宇 韩南银 

机构地区：北京大学药学院,北京100191

出　　处：《色谱》2017年第2期216-221,共6页Chinese Journal of Chromatography

摘　　要：重力场流分离是最简单的场流分离（ gravitational flow-field fractionation, GrFFF）技术,常用于分离粒径几微米到几十微米的颗粒及生物样品.利用自组装加工的重力场流分离仪器分离3种不同粒径（3、6、20μm）的聚 苯乙烯（PS）颗粒.自制了一种混合表面活性剂,并与商品化的表面活性剂FL-70进行了比较.通过均匀设计优化 流速、混合表面活性剂中聚乙二醇辛基苯基醚（Triton X-100）的质量分数、载液黏度、停流时间等分离条件,以分离 度（Rs）和保留比（R）为评价指标,发现FL-70的分离效能略优于自制的混合表面活性剂,可实现3种PS颗粒的完全分离（Rs1,为1. 771,Rs2为2. 074）.结果表明该系统具有良好的分离性能.Gravitational flow-field fractionation (GrFFF) is the simplest and cheapest field. flow fractionation technique. GrFFF utilizes earth's gravity as external field, providing a size. based separation. It can be applied to the separation and characterization of various micrometer. sized particles and biological samples. A GrFFF device was assembled, and three kinds of polystyrene (PS) particles with different sizes (20, 6 and 3 mu m) were used as the model particles. A homemade mixed surfactant was used and was compared with the surfactant FL-70. The separation conditions of GrFFF were optimized with uniform design, including flow rate, mass percentage of polyethylene glycol octylphenol ether (Triton X-100) in mixed surfactant, viscosity of carrier liquid and stop. flow time. The resolution (R-s) and retention ratio (R) were utilized as the evaluation indexes. The results suggest that the separation performance of FL-70 was slightly better than that of the mixed surfactant. Using FL-70 as the surfactant, particles of 20, 6 and 3 mu m were completely separated (with resolutions of 1.. 771 and 2.. 074). The results indicate that this system has good separation performance.

关 键 词：重力场流分离 均匀设计 聚苯乙烯颗粒 载液 表面活性剂 流速 分离度 

分 类 号：O658[理学—分析化学]