双糖保护剂对胰岛素活性结构稳定性的影响  

Bioactive Structural Stability of Insulin in Disaccharides Solutions

作　　者：孙旭[1] 李代禧[1] 郭柏松 

机构地区：[1]上海理工大学医疗器械与食品学院,上海200093 [2]上海东富龙科技股份有限公司,上海201108

出　　处：《上海理工大学学报》2016年第6期598-604,共7页Journal of University of Shanghai For Science and Technology

基　　金：上海市重点学科建设资助项目(T0503;P0502);上海市自然科学基金资助项目(12ZR1420400);上海市"创新行动计划"国际科技合作项目(12430702000);上海市联盟计划项目(11XSY23)

摘　　要：采用微量热分析法并结合分子动力学模拟,研究了不同双糖保护剂对胰岛素的保护效果.结果表明,通过区域选择性吸附,双糖保护剂可以在胰岛素表面产生保护作用,提高胰岛素的热稳定性.在4种双糖保护剂中,吸附作用越强的保护剂对胰岛素热稳定性的提升效果越好.随着保护剂质量分数的增加,胰岛素的热稳定性呈先增强后稳定的趋势.进一步分析了保护剂与水分子之间的相互作用,得出双糖保护剂与胰岛蛋白之间的直接相互作用可能是提高其热稳定性的主要原因.The approaches of microcalorimetry and molecular dynamic simulation were adopted to investigate the protective effect caused by adding protectants.The results indicate that disaccharide protectants are able to maintain the molecular structure of insulin and contribute to the thermal stability of insulin due to the regional selective adsorption of protectants on the surface of insulin.Among four disaccharide protectants,the protectant with stronger adsorption ability conducts better performance in stabilizing the molecular structure of protein.With increasing the concentration of protectants,the thermal stability of insulin changes from increasing to gradually stable state.A further analysis upon the interaction between protectants and water reflects that increasing the thermal stability of insulin is attributed mainly to the interaction between insulin and disaccharides.

关 键 词：双糖 胰岛素 热稳定性 分子模拟 微量热 

分 类 号：O6-39[理学—化学]