葛根素在卵巢早衰患者中的免疫调节作用  

Effect of Puerarin on the immune regulation of patients with premature ovarian failure

作　　者：张金慧[1] 杜洪灵[1] 周琼青[1] ZHANG Jin-hui DU Hong-ling ZHOU Qiong-qing(Department of Obstetrics and Gynecology, Putuo District People？s Hospital, Shanghai 200060, Chin)

机构地区：[1]上海市普陀区人民医院妇产科，上海 200060

出　　处：《中国临床医学》2016年第6期816-819,共4页Chinese Journal of Clinical Medicine

基　　金：上海市普陀区科学技术委员会基金(KW15201)~~

摘　　要：目的:探讨葛根素(Puerarin)对卵巢早衰(premature ovarian failure,POF)患者的免疫调节机制。方法:随机选取抗卵巢抗体(AoAb)阳性的POF患者40例,采用葛根素-安宫黄体酮序贯治疗。在治疗前和治疗第12个周期采用Kupperman评分法评价该方法的临床疗效;并检测患者AoAb,CD3^+、CD4^+、CD8^+T淋巴细胞,CD4^+/CD8^+值,CD19^+B淋巴细胞,抗核抗体(ANA)等指标。统计治疗过程中不良反应的发生情况。结果:治疗后患者的Kupperman评分、ANA转阴率、AoAb转阴率和AoAb浓度降低(P<0.05)。治疗后CD8^+T细胞数明显增加;而CD3^+、CD4^+T淋巴细胞,CD4^+/CD8^+比值,CD19^+B淋巴细胞数明显减少或降低,差异均有统计学意义(P<0.05)。Kupperman评分改变与AoAb,CD3^+、CD4^+T淋巴细胞数,CD19^+B淋巴细胞数,ANA变化正相关(P<0.05);Kupperman评分与CD8^+T细胞计数负相关(P<0.05)。结论:葛根素-安宫黄体酮序贯疗法可能通过调节CD3^+、CD4^+、CD8^+T淋巴细胞功能来调节机体免疫功能,通过抑制CD19^+B淋巴细胞的增殖及ANA、AoAb等的分泌来消除POF的免疫性损伤,从而实现卵巢的自我修复和功能恢复。Objective ： To discuss the immune regulation mechanism of Puerarin in patients with premature ovarianfailure （ P O F） , and to study the anti-ovary anti-body （A o A b ）, antinuclear antibody （ AN A） ,CD 3＋,CD 4＋,CD8＋ Tlymphocyte cell and CD4＋ /CD8＋ and changes of CD19 ＋ B lymphocyte cell before and after treatment. Methods ： Totally 40POF cases with AoAb positive, treated with Puerarin-progesterone sequential treatment, were random selected. The clinicalefficacy was analyzed before treatment and at the 12th cycle of treatment by using Kupperman, and the AoAb, CD 3＋,CD4＋ ,CD8＋ T cells and CD4＋/CD8＋, CD19＋ B lymphocyte cell, ANA and other indicators were recorded and analyzed. Theincidence rate of adverse reactions in the treatment process was recorded. Results： Kupperman score, ANA negative rate,AoAb negative rate and concentration of antibody after treatment were significantly decreased （P〈C〇. 05）. The CD8＋ T cellcounts increased significantly, while the CD 3＋,CD4＋ T cells, CD4＋/CD8＋,CD19＋B lymphocytes were significantly reducedafter treatment （P〈C〇. 05）. Kupperman score was positively correlated with the number of AoAb, CD 3＋,CD4＋ T cells,CD19＋ B cell count, and ANA （P〈C0. 0 5 ）, and negative correlated with CD8＋ T cell count （P〈C0. 05）. Conclusions： Puerarinprogesteronesequential treatment is a safe and effective method for the POF. Puerarin may regulate immune function throughthe CD 3＋,CD4＋ and CD8＋T cells, eliminate the immune injury of the POF by inhibiting the CD19＋B cell, and secretion ofANA and AoAb to realize the self-repairing and functional recovery of ovarian

关 键 词：卵巢早衰 葛根素-安宫黄体酮序贯疗法 抗卵巢抗体 抗核抗体 T淋巴细胞 B淋巴细胞 

分 类 号：R711.75[医药卫生—妇产科学]