骨关节炎患者关节液中miR-335-5p表达及意义  

Expression and significance of miR-335-5p in the synovial fluid of patients with osteoarthritis

作　　者：何伟珍[1] 尹志华[1] 郭佩[1] 徐晓东[1] 叶志中[1] 

机构地区：[1]深圳市第四人民医院香蜜湖风湿病分院,广东医学院深圳风湿病研究所,深圳518040

出　　处：《新医学》2017年第1期37-39,共3页Journal of New Medicine

基　　金：国家自然科学基金(81102266,81301529);深圳市科技计划项目(JCYJ20140416094256891)

摘　　要：目的检测miR-335-5p在骨关节炎患者关节液中的表达水平,分析miR-335-5p与Kellgren-Lawrence X线分级之间的关系。方法实时定量PCR检测20例骨关节炎患者和20例非骨关节炎患者膝关节液中miR-335-5p的表达水平,对骨关节炎患者膝关节进行Kellgren-Lawrence X线分级,分析miR-335-5p的表达水平与膝关节Kellgren-Lawrence X线分级的相关性。结果 miR-335-5p在骨关节炎患者关节液的△Ct值为9.78±2.50,非骨关节炎患者关节液的△Ct值为8.23±2.13,miR-335-5p在骨关节炎患者关节液的表达水平低于非骨关节炎患者(P<0.05)。骨关节炎患者miR-335-5p在关节液中相对表达量与Kellgren-Lawrence X线分级之间无关(rs=-0.351,P=0.062)。结论miR-335-5p在骨关节炎患者关节液中表达下调,提示其与骨关节炎的发生、发展可能有关。Objective To measure the expression level of miR-335-5p in the synovial fluid of pa-tients with osteoarthritis （OA） and analyze the relationship between miR-335-5p and Kellgren-Lawrence classi-fication grade. Methods The expression levels of miR-335-5p in the synovial fluid of OA patients （n = 20） and non-OA controls （n = 20） were detected using quantitative real-time PCR （qRT- PCR）. Kellgren-Lawrence classification of the knee joint was evaluated. The correlation between miR-335-5p level and Kellgren-Lawrence classification grade was assessed. Results Down-regulated miR-335-5p was observed in synovial fluid of OA patients （ △Ct 9.78 ±2. 50） compared with that in non-OA controls （ △Ct 8.23 ± 2.13） （P 〈0.05）. No correlation was observed between miR-335-5p level and Kellgren-Lawrence classification grade （ rs = - 0.351, P =0.062）. Conclusion The expression level of microRNA-335-5p is down-regulated in the synovial fluid of OA patients, suggesting that it is probably correlated with the incidence and progression of OA.

关 键 词：骨关节炎 微小核糖核酸335-5p 骨赘形成 

分 类 号：R684.3[医药卫生—骨科学]