U-shaped microRNA expression pattern could be a new concept biomarker for environmental estrogen  

作　　者：Rui Duan Yun Lu Lingyan Hou Lina Du Lequn Sun Xingfan Tang 

机构地区：[1]State Key Joint Laboratory of Environment Simulation and Pollution Control, School of Environment, Tsinghua University, Beijing 100084, China

出　　处：《Frontiers of Environmental Science & Engineering》2016年第6期93-101,共9页环境科学与工程前沿（英文）

基　　金：Acknowledgements This work was financially supported by the National Natural Science Foundation of China （Grant Nos. 21377066 and 21007030）.

摘　　要：Many studies have focused on environmental estrogen-related diseases. However, no consistent gene markers or signatures for estrogenicity have been discovered in mammals. This study investigated the estrogenic effects of 17β-estradiol on the prostate in immature male mice. Consistent U-shaped responses were seen in bodyweight, ventral prostate epithelial morphology, and miRNA expression levels. Specifically, most estmdiol regulated miRNAs were downregulated at low doses of estradiol （0.2 and 2 μg. kg-1）, and whose expression returned to the control level at a larger dose （200 μg-kg-1）. The fi.mction of these regulated miRNAs is related to the prostate cancer and PI3K-Akt signaling pathways, which is consistent with the function of estmdiol. Furthermore, the miRNA-processing machinery, Drosha, in the prostate was also regulated in a similar pattern, which could be a part of the U-shaped miRNA expression mechanism. All of these data indicate that the prostate is a reliable organ for evaluating estrogenic activity and that the typical nonmonotonic dose-response relationship could be used as a novel biomarker for estrogenicity.Many studies have focused on environmental estrogen-related diseases. However, no consistent gene markers or signatures for estrogenicity have been discovered in mammals. This study investigated the estrogenic effects of 17β-estradiol on the prostate in immature male mice. Consistent U-shaped responses were seen in bodyweight, ventral prostate epithelial morphology, and miRNA expression levels. Specifically, most estmdiol regulated miRNAs were downregulated at low doses of estradiol （0.2 and 2 μg. kg-1）, and whose expression returned to the control level at a larger dose （200 μg-kg-1）. The fi.mction of these regulated miRNAs is related to the prostate cancer and PI3K-Akt signaling pathways, which is consistent with the function of estmdiol. Furthermore, the miRNA-processing machinery, Drosha, in the prostate was also regulated in a similar pattern, which could be a part of the U-shaped miRNA expression mechanism. All of these data indicate that the prostate is a reliable organ for evaluating estrogenic activity and that the typical nonmonotonic dose-response relationship could be used as a novel biomarker for estrogenicity.

关 键 词：miRNA Prostate Estradiol Nonmonotonic dose-response Estrogenicity Drosha 

分 类 号：T[一般工业技术]