肠炎清对免疫复合溃疡型结肠炎模型大鼠结肠组织NF-κB蛋白表达和TLR4、MyD88基因表达的影响  

The effect of Changyanqing decoction on the expression of NF-κB protein,TLR4 and MyD88 gene in colonic tissue of ICUC rats

作　　者：黄亦彤[1] 钟志勇[2] 吕永慧[1] 武昕[2] 潘竞锵[1] 蓝天美 严家荣[2] HUANG Yi-tong ZHONG Zhi-yong LV Yong-hui WU Xin PAN J ing-qiang LAN Tian-mei YAN J ia-rong(Department of Pharmaceutical, The Chinese Medicine Hospital in Guangzhou, Guangzhou 510000, China Medical Experimental Animal Center of Guangdong Province, Guangzhou 510000, China)

机构地区：[1]广州市中医医院药学部,广东广州510000 [2]广东省医学实验动物中心,广东广州510000

出　　处：《中国中西医结合消化杂志》2016年第12期906-910,915,共6页Chinese Journal of Integrated Traditional and Western Medicine on Digestion

基　　金：广州市医药卫生科技项目中医药科技项目(No:20132A011012)

摘　　要：[目的]观察肠炎清对免疫复合溃疡型结肠炎模型(ICUC)大鼠结肠组织核因子-κB(NF-κB)蛋白表达及Toll样受体4(TLR4)、髓样分化因子88(MyD88)基因表达的影响,探讨其治疗IBD的作用机制。[方法]采用三硝基苯磺酸(TNBS)加免疫复合法造模。将大鼠随机分为空白对照组、模型对照组、肠炎清低、中、高剂量组(8.33、16.67、33.33ml/kg)、柳氮磺吡啶(SASP)组(0.5g/kg)。造模后第2天,用药组分别给予相应药物灌胃,7d后,麻醉处死大鼠,取新鲜结肠标本。采用免疫组化法检测NF-κBp65的蛋白表达,实时定量PCR法(qRT-PCR)检测TLR4mRNA、MyD88mRNA的表达。[结果]免疫组化结果 NF-κBp65在各组各只动物均可见阳性表达,其中肠炎清高剂量组及SASP组可显著下调NF-κBp65的表达,差异有统计学意义(P<0.05)。PCR结果:造模后动物结肠组织TLR4mRNA、MyD88mRNA的表达量均有升高。与模型对照组比较,各给药组TLR4 mRNA、MyD88mRNA表达均有不同程度的下调,但差异无统计学意义。[结论]肠炎清能下调NF-κBp65蛋白表达、TLR4、MyD88基因表达,其作用机制可能与调控TLR4/MyD88/NF-κB信号转导通路有关。[Objective]To observe the expression of NF-κB and the expression of TLR4 and MyD88gene in colonic tissue of rats with immune complex ulcerative colitis（ICUC）And explore the mechanism of IBD treatment.[Methods]Trinitrobenzene sulfonic acid（TNBS）plus immune complex method were used to make the rats modle which were randomly divided into blank control group,model control group,enteritis of low,medium and high dose group（8.33,16.67,33.33ml/kg）,willow nitrogen sulfonyl pyridine（SASP）group（0.5g/kg）.After 2days,The treatment group rats were sacrificed after administrating the corresponding drugs for 7days,and fresh colon specimens were taken.The protein expression of NF-κBp65was detected by immunohistochemical method and the expression of TLR4 mRNA and MyD88 mRNA was detected by RT-PCR.[Results]Immunohistochemical results：The nf-kappa Bp65 positive expression,appear in both groups of various animals including enteritis lofty dose group and SASP group significantly lowered the nf-kappa Bp65 expression,statistically significant（P〈0.05）.The PCR results：The expressions of TLR4 mRNA and MyD88 mRNA in the colon tissue of the animals were increased after modeling.Compared with model control group,TLR4 mRNA and MyD88 mRNA expression were down-regulated in different dose groups,but there was no significant difference.[Conclusion]Changyanqing decoction can decrease the expression of nf-kappa Bp65 protein and the TLR4,MyD88 gene.Its mechanism may be related to the regulation of-TLR4/MyD88/nf-kappa B signal transduction pathway.

关 键 词：肠炎清 溃疡性结肠炎 ICUC大鼠 NF-ΚBP65 TLR4 MRNA MYD88 MRNA 

分 类 号：R285.5[医药卫生—中药学]