神经调节蛋白1对冠状动脉平滑肌细胞表达血管生成因子的影响  

Neuregulin-1 induces expression of angiogenic factors in coronary artery smooth muscle cells

作　　者：庞毅恒 桂春[1] 陈力铨 

机构地区：[1]广西医科大学第一附属医院西院心血管内科,广西南宁530021

出　　处：《中国病理生理杂志》2016年第11期1945-1948,共4页Chinese Journal of Pathophysiology

基　　金：国家自然科学基金资助项目(No.81160021;No.81460063);广西自然科学基金重点课题(No.2014GXNSFDA118024)

摘　　要：目的:探讨神经调节蛋白1(neuregulin-1,NRG-1)对人冠状动脉平滑肌细胞(HCASMCs)表达血管生成因子的影响。方法:培养HCASMCs,实验使用第3代细胞。用Western blot法检测细胞Erb B的表达和磷酸化。在正常、缺氧缺血清或NRG-1(100μg/L)处理条件下,用Western blot法检测血管内皮生长因子(VEGF)、血管生成素1(Ang-1)和血管生成素2(Ang-2)表达的改变。结果:Erb B2、Erb B3和Erb B4均能在HCASMCs中表达,加入NRG-1后,这3种Erb B的磷酸化水平均增加。与对照组比较,在缺氧缺血清组HCASMCs中VEGF和Ang-1的表达明显增加(P<0.05),而Ang-2的表达差异无统计学显著性。与缺氧缺血清组比较,NRG-1处理组的HCASMCs表达VEGF和Ang-1进一步显著增加(P<0.05),而Ang-2的表达差异无统计学显著性。结论:HCASMCs能表达Erb B2、Erb B3和Erb B4,加入NRG-1增强Erb B2、Erb B3和Erb B4的磷酸化。缺氧缺血清和NRG-1处理均能增加VEGF和Ang-1在HCASMCs中的表达。AIM： To investigate the effects of neuregulin-1（ NRG-1） on the expression of angiogenic factors in human coronary artery smooth muscle cells（ HCASMCs）. METHODS： HCASMCs were cultured in vitro,and the cells at the 3rd passage were collected to assess the expression and phosphorylation of Erb B by Western blot. After HCASMCs were cultured under normal condition,with hypoxia and serum deprivation,or with NRG-1 treatment,the expression of vascular endothelial growth factor（ VEGF）,angiopoietin-1（ Ang-1） and angiopoietin-2（ Ang-2） was determined by Western blot.RESULTS： The expression of Erb B2,Erb B3 and Erb B4 was observed in the HCASMCs,and the phosphorylation of these receptors was increased by NRG-1 treatment. Compared with control group,the expression of VEGF and Ang-1 in the HCASMCs was significantly increased in hypoxia and serum deprivation group（ P〈0. 05）,while no difference in the expression of Ang-2 between the 2 groups was found. Compared with hypoxia and serum deprivation group,the expression of VEGF and Ang-1 in the HCASMCs treated with NRG-1 was further increased（ P〈0. 05）,and no difference in the expression of Ang-2 between the 2 groups was observed. CONCLUSION： HCASMCs express Erb B2,Erb B3 and Erb B4,and the phosphorylation of the receptors is increased by NRG-1. Hypoxia,serum deprivation and NRG-1 treatment induce the increased expression of VEGF and Ang-1 significantly.

关 键 词：神经调节蛋白1 人冠脉平滑肌细胞 血管内皮生长因子 血管生成素 

分 类 号：R543.3[医药卫生—心血管疾病] R363[医药卫生—内科学]