原因不明性肝炎血清中TTVDNA的检出及其临床意义  

A study on TTV DNA detection and clinical significance in patients with hepatitis of unknown etiology

作　　者：潘发愤[1] 陶志华[1] 陈晓东[1] 王忠永[1] 周祖木[2] 吴建波[1] 丁建光 陈永平[1] 王震龙[1] 

机构地区：[1]温州医学院附属第一医院,浙江温州325000 [2]温州市疾病预防控制中心,浙江温州325000 [3]瑞安市人民医院,浙江瑞安325200

出　　处：《临床肝胆病杂志》2002年第4期228-229,共2页Journal of Clinical Hepatology

基　　金：温州市科委医学科学基金资助 (S2 0 0 0A17)

摘　　要：研究原因不明性肝炎中TTV (Transfusiontransmittedvirus)感染的检出状况及其临床意义。在TTVORF1设计引物 ,建立巢式聚合酶链反应 (Nested -PCR) ,检测 5 0例原因不明性肝炎、4 0例正常健康人与 30例乙肝病毒携带者血清中TTVDNA ,比较TTVDNA阳性肝炎患者临床特征。 5 0例原因不明性肝炎中 16例TTVDNA阳性 (32 % ) ,比正常健康人群 (2 / 4 0 ,5 % )与乙肝病毒携带者 (2 / 30 ,6 7% )血清中TTVDNA检出率为高(P <0 0 5 ) ,16例TTVDNA阳性患者中以慢性肝炎占多数 (12 / 16 ,75 5 % ) ,表现为反复、持续ALT异常。TTV在原因不明性肝炎中感染率明显高于其他人群 。To investigate TTV infection among patients with hepatitis of unknown etiology and its clinical significance. A nested polymerase chain reaction (PCR) assay with primers from ORF1 of TTV genome was established to detect TTV DNA in 50 patients with hepatitis of unknown etiology, 40 health persons and 30 HBV carriers. The positive rate of TTV DNA in patients with hepatitis of unknown etiology, health persons and in HBV carriers were 32%(16/50), 5%(2/40)and 6 7%(2/30), respectively. It had statistical difference between them ( P <0 05). Among 16 patients with TTV DNA positive response, 12 cases were chronic hepatitis (12/16,75%). TTV infection would be one of the major causes of hepatitis with unknown etiology in this areas.

关 键 词：原因不明性肝炎 血清 TTVDNA 临床意义 巢式聚合酶链反应 

分 类 号：R512.6[医药卫生—内科学]