旋毛虫肌幼虫排泄分泌抗原诊断早期旋毛虫病的研究  

Early serodiagnosis of trichinellosis by Western blot with excretory-secretory antigens from Trichinella spiralis muscle larvae

作　　者：文慧[1] 胡晨曦[1] 王李昂 王涵[1] 刘春颖[1] 宋艳艳[1] 刘若丹[1] 姜鹏[1] 王中全[1] 崔晶[1] WEN Hui HU Chen-xi WANG Li-ang WANG Han LIU Chun-ying SONG Yan-yan LIU Ruo-dan JIANG Peng WANG Zhong-quan CUI Jing(Department of Parasitology, Basic Medical College, Zhengzhou University, Zhengzhou 450052, Chin)

机构地区：[1]郑州大学医学院寄生虫学教研室,郑州450052

出　　处：《中国人兽共患病学报》2016年第12期1058-1063,共6页Chinese Journal of Zoonoses

基　　金：国家自然科学基金项目(No.81672043);河南省高等学校科研项目(No.16A310017)联合资助~~

摘　　要：目的评价旋毛虫肌幼虫排泄分泌(excretory-secretory,ES)抗原诊断早期旋毛虫病的价值。方法应用旋毛虫肌幼虫ES抗原Western blot对旋毛虫感染后6~11d的小鼠血清及19d的早期旋毛虫病人血清进行检测,并与感染后35d的晚期旋毛虫病人和其他寄生虫病人血清的检测结果进行比较。结果 Western blot分析显示,肌幼虫ES蛋白中的2条蛋白带(41.5、55kDa)可被旋毛虫感染后7~11d的小鼠血清识别,6条蛋白带(41.5、44.1、45、55、61和65.2kDa)能被早期和晚期旋毛虫病人血清识别,阳性反应率均为100%(15/15);这6条蛋白带与裂头蚴病人和健康人血清无交叉反应,但与其他寄生虫病(血吸虫病、并殖吸虫病、华支睾吸虫病、棘球蚴病及囊尾蚴病)患者血清具有明显的交叉反应(19.12%~38.23%)。结论旋毛虫肌幼虫ES抗原中的41.5kDa^65.2kDa蛋白带可与旋毛虫感染早期血清反应,但与其他蠕虫病患者有明显的交叉反应。To evaluate the value of Trichinella spiralis muscle larval excretory-secretory（ES）antigens for early serodiagnosis of trichinellosis,the larvae were cultured in RPMI-1640 at 37 ℃in 5% CO2 for 18hand the ES antigens were analyzed by SDS-PAGE.Western blot with T.spiralis muscle larval ES antigens was used to detect the serum samples from mice infected with T.spiralis at 6-11 days post infection（dpi）and the patients with trichinellosis at 19 dpi,and the results were compared with those of serum samples from patients with trichinellosis at 35 dpi and other parasitic diseases.The results of SDSPAGE indicated that T.spiralis muscle larval ES antigens had 19 protein bands with a molecular weight of 15-97 kDa,whereas the results of Western blot analysis showed that two protein bands（41.5and 55kDa）in the ES antigens were recognized by mouse infection sera at 7-11 dpi,and six protein bands（41.5,44.1,45,55,61 and 65.2kDa）were recognized by serum samples from early and late patients with trichinellosis,and the positive reaction rate was 100%（15/15）.The six proteins had no cross-reaction with serum samples of patients with sparganosis and healthy individuals,but they had obvious cross reaction with serum samples from the patients with other parasitic diseases（schistosomiasis,paragonimiasis,clonorchiasis,echinococcosis,and cysticercosis）,with the cross reaction rate ranging from 19.12%to 38.23%.The results indicate the six protein bands with 41.5-65.2kDa in T.spiralis muscle larval ES antigens are positively reacted with early infection sera,but they obvious have cross reaction with sera from the patients with other helminthinasis.

关 键 词：旋毛虫病 旋毛虫 肌幼虫 排泄分泌抗原 Western BLOT 早期诊断 

分 类 号：R383.15[医药卫生—医学寄生虫学]