鰤鱼诺卡氏菌感染斑马鱼模型的建立与组织病理学研究  

Establishment of a Zebrafish Model for Nocardia seriolae and Histopathological Study

作　　者：夏立群[1,2,3] 汪美[1] 赖杰彬 陈锐敏[1] 徐亮[1] 鲁义善[1,2,3] XIA Liqun WANG Mei LAI Jiebin CHEN Ruimin XU Liang LU Yishan(College of Fisheries, Guangdong Ocean University, Zhanjiang, Guangdong 524088, China Guangdong Key Laboratory of Pathogenic Biology and Epidemiology for Aquatic Economic Animals, Zhanjiang, Guangdong 524088, China Guangdong Higher Education Institutions Key Laboratory for for Controlling of Diseases of Aquatic Economic Animals, Zhanjiang, Guangdong 524088, China)

机构地区：[1]广东海洋大学水产学院,广东湛江524088 [2]广东省水产经济动物病原生物学及流行病学重点实验室,广东湛江524088 [3]广东省高等学校水产经济动物病害控制重点实验室,广东湛江524088

出　　处：《热带生物学报》2016年第4期409-416,共8页Journal of Tropical Biology

基　　金：广东省科技发展专项资金项目(2016A050502061);广东省教育厅特色创新项目(2014KTSCX075);广东省团委攀登计划项目(pdjh2016b0234);"海之帆-起航计划"大学生科技创新培育专项(hzfqhjhzrkx2015b03)

摘　　要：鱼类诺卡氏菌病近年来在我国南方省份不断蔓延,对水产行业造成巨大影响,鰤鱼诺卡氏菌(Nocardia seriolae)是鱼类诺卡氏菌病的主要病原,危害约20种海、淡水养殖鱼类。本研究以斑马鱼为模式动物,腹腔接种不同稀释度的强毒株鰤鱼诺卡氏菌ZJ0503,通过临床症状观察、病理剖检、病原菌分离鉴定、Kaplan-Meier生存曲线分析、半数致死剂量(LD_(50))统计和组织病理学研究,初步建立了鰤鱼诺卡氏菌-斑马鱼模型。研究发现攻毒后斑马鱼出现明显的发病症状,鰤鱼诺卡氏菌ZJ0503对斑马鱼的LD50为3.39×106CFU·尾,斑马鱼感染后,肾脏、肝脏、胰脏、鳃、皮肤和肌肉等组织出现明显病理学变化。结果表明,斑马鱼是鰤鱼诺卡氏菌良好的动物感染模型,该动物模型的建立将有助于诺卡氏菌病致病机理的研究和疫苗的研制。Fish nocardiosis has been spread in the south of China in recent years and has a huge adverse effect on the fishery industry. Nocardia seriolae is a leading cause of fish nocardiosis and has been reported in about 20 fish species. Zebrafish（ Danio rerio） were infected with a strong virulent？ strain of N. seriolae ZJ0503 at different concentrations through intraperitoneal injection. A zebrafish model for N. seriolae was established through the observation of clinical symptoms,pathologic autopsy,pathogen isolation,50% lethal dose（ LD50） analysis and histopathological studies. The results show that an obvious infection happened in the zebrafish after challenged with N. seriolae ZJ0503. The LD50 of N. seriolae ZJ0503 was 3. 39 × 10^8 CFU/fish. Obvious pathological changes were observed in the zebrafish tissues,such as kidney,liver,pancreas,gill,skin and muscle. This study provides evidence that the zebrafish can be used as a model for N. seriolae infection,and the model is useful to study the pathogenic mechanism of fish nocardiosis for development of N. seriolae vaccine.

关 键 词：鰤鱼诺卡氏菌 斑马鱼 动物模型 组织病理 

分 类 号：S941[农业科学—水产养殖]