农杆菌介导的木薯遗传转化体系的优化  

The Optimization System for Agrobacterium-mediated Genetic Transformation of Cassava

作　　者：廖华兰 黎秀琼[1] 李可[1] 李伯凌 熊茜[1] 苏童[1] 李春霞[1] 陈银华[1] 罗丽娟[1] LIAO HuaLan LI XiuQiong LI Ke LI BoLing XIONG XI SU Tong LI ChunXia CHEN YinHua LUO LiJun(The Agricultural College of Hainan University/Hainan Key Laboratory for Sustainable Utilization of Tropical Bioresources, Hainan University, Haikou, Hainan 570228,Chin)

机构地区：[1]海南大学农学院/海南省热带生物资源可持续利用重点实验室,海口570228

出　　处：《热带生物学报》2016年第4期427-434,共8页Journal of Tropical Biology

基　　金：国家自然科学基金(31260345;31560497);海南省重大科技项目(ZDZX2013023-2)

摘　　要：以木薯腋芽为外植体,通过添加不同含量的2,4-D和picloram摸索诱导体细胞胚及FEC的最佳激素条件;利用优化的再生体系,以木薯脆性胚性愈伤组织(FEC)为受体,对农杆菌侵染FEC的侵染浓度、侵染时间以及侵染后潮霉素抗性筛选浓度进行了研究。结果表明,12 mg·L^(-1)的picloram能较快诱导出体细胞胚及FEC;农杆菌菌液浓度OD600=0.4并侵染30 min为FEC转化效率及植株再生率的最佳条件;共培养后,通过逐渐增加潮霉素筛选浓度(0,5,8,15 mg·L^(-1))能更有效地提高植株的再生率。Cassava axillary buds was used as explants,by adding different concentrations of 2,4-D and picloram to explore the best hormone condition for inducing somatic embryos and FEC; Using optimized regeneration system,studying the infection concentration、infection time of of Agrobacterium and the Screening concentration of hygromycin. The results show that： 12 mg·L^-1 picloram is the best hormone concentration to induction somatic embryo and FEC; the optimal conditions for the conversion efficiency of FEC and the plant regeneration is OD600= 0. 4 of Agrobacterium and 30 min infection time,By increasing the concentration of hygromycin（ 0,5,8,15 mg·L^-1） selection is more advantageous to plant regeneration. The results of the study have optimized the existing cassava genetic transformation system,which provides a platform for cassava genetic engineering breeding.

关 键 词：木薯 组织培养 报告基因 遗传转化 分子鉴定 

分 类 号：S533[农业科学—作物学]