七氟醚预处理对小鼠Lewis肺癌细胞肺转移的抑制作用  

Effects of sevoflurane pretreatment on lung metastasis of mouse Lewis lung cancer cells

作　　者：徐枫[1] 张涛[1] 郑雪琴[1] 杨承祥[1] 梁桦[1] 

机构地区：[1]佛山市第一人民医院麻醉科,528000

出　　处：《临床麻醉学杂志》2017年第1期71-73,共3页Journal of Clinical Anesthesiology

摘　　要：目的观察七氟醚预处理对小鼠Lewis肺癌细胞肺转移的影响。方法小鼠Lewis肺癌细胞接种于培养板,培养24h后,采用随机数字表法,将其随机分为四组:对照组(CC组)、1%七氟醚组(SC1组)、2%七氟醚组(SC2组)和3%七氟醚组(SC3组)。CC组不接受七氟醚处理,SC1~3组细胞分别用1%、2%、3%七氟醚处理4h,继续培养24h。采用Transwell法检测细胞侵袭能力,细胞划痕实验检测细胞迁移能力;采用ELISA法检测细胞MMP-2和MMP-9蛋白表达。32只C57BL/6小鼠随机分为四组:对照组(CM组)、1%七氟醚组(SM1组)、2%七氟醚组(SM2组)和3%七氟醚组(SM3组),每组8只。取SC1~3组七氟醚预处理后的Lewis肺癌细胞悬液0.1ml(2×107个/ml),分别给SM1~3组小鼠尾静脉注射,CM组小鼠注射等体积的CC组细胞悬液。3周后观察肺部转移灶,计算肺转移抑制率。结果 SC1~3组细胞侵袭能力和迁移能力明显低于CC组,且SC1组>SC2组>SC3组(P<0.05);SC1~3组MMP-2和MMP-9浓度明显低于CC组,且SC1组>SC2组>SC3组(P<0.05);SM1~3组肺转移抑制率明显高于CM组,且SM1组<SM2组<SM3组(P<0.05)。结论七氟醚预处理小鼠Lewis肺癌细胞可抑制其在小鼠体内的肺转移,此效应可能与下调小鼠Lewis肺癌细胞MMP-2和MMP-9表达有关。Objective To observe the effects of sevoflurane pretreatment on lung metastasis of mouse Lewis lung cancer（LLC）cells.Methods Mouse LLC cells were inoculated in culture plate.After being cultured for 24 hthe cells were randomly divided into four groups：group control（CC）,group 1% sevoflurane（SC1）,group 2% sevoflurane（SC2）,and group 3% sevoflurane（SC3）.Cells of group SC1-3were exposed to 1%,2%,3% sevoflurane for 4hrespectively,cells of group CC were exposed to 95%O2-5%CO2 mixture air,and were then cultured for another 24 h.The invasive activity of cells was determined by Transwell assay.The migration of cells was evaluated by wound scratch assay.The expression of MMP-2and MMP-9in cells were detected by ELISA.Thirty-two C57BL/6mice were divided into four groups（n=8）：group control（CM）,group 1% sevoflurane（SM1）,group 2% sevoflurane（SM2）,and group 3% sevoflurane（SM3）.LLC cells of group SC1-3were injected into caudal vein of mouse in group SM1-3respectively.Cells of group CC were injected into mouse of group CM.Lung metastasis inhibitory rates were evaluated after 3 weeks.Results Compared with group CC,the invasive activity and migration of cells in group SC1-3were decreased significantly,group SC〉1group SC2〉group SC3（P〈0.05）;the protein expression of MMP-2and MMP-9was significantly down-regulated with sevoflurane concentration increased,group SC1〉group SC2〉group SC3（P〈0.05）.Compared with group CM,lung metastasis inhibitory rates of group SM1-3 were increased significantly,group SM1〈group SM2〈group SM3（P〈0.05）.Conclusion Sevoflurane can inhibit the lung metastasis of mouse LLC cells,which maybe through down-regulating the expression of MMP-2and MMP-9in mouse LLC cells.

关 键 词：七氟醚 肺转移 LEWIS肺癌 

分 类 号：R614[医药卫生—麻醉学] R734.2[医药卫生—外科学]