毁灭炭疽菌Colletotrichum destructivum诱抗蛋白对烟草抗病性的诱导  

Induced resistance of tobacco by the protein elicitor of Colletotrichum destructivum

作　　者：田华[1,2] 朱艳梅[2] 董瑜 刘伟[1] 孔凡玉[1] 王静[1] 

机构地区：[1]中国农业科学院烟草研究所,中国烟草总公司青州烟草研究所烟草行业病虫害监测与综合治理重点实验室,山东青岛266101 [2]云南省昆明市烟草公司石林分公司,石林652200 [3]云南省曲靖市烟草公司会泽分公司,会泽654200

出　　处：《植物保护学报》2017年第1期67-74,共8页Journal of Plant Protection

基　　金：福建省南平市科技项目(201203)

摘　　要：为明确毁灭炭疽菌Colletotrichum destructivum诱抗蛋白诱导烟草的抗病性及其作用,采用喷雾、摩擦接种方法及RT-PCR技术研究了诱抗蛋白的预防保护作用,以及烟草悬浮细胞经诱导后过氧化物酶(POD)、多酚氧化酶(PPO)、苯丙氨酸解氨酶(PAL)活性及脯氨酸(Pro)含量和病程相关基因表达的变化。结果表明,接种3、5和7 d后,该诱抗蛋白对烟草炭疽病的诱抗效果分别为58.00%、48.99%和49.65%,对烟草白粉病的诱抗效果分别为83.26%、80.76%和78.60%,并可以抑制烟草普通花叶病毒的复制及在寄主体内的扩增;经诱抗蛋白处理后,烟草悬浮细胞POD、PPO、PAL活性及Pro含量明显提高;诱抗蛋白能够诱导烟草病程相关蛋白基因PR-1a、PR-1b以及抗病信号传导途径关键基因NPR1的表达。表明毁灭炭疽菌诱抗蛋白可诱导烟草产生抗病性,可能与烟草悬浮细胞中POD、PAL、PPO的活性及Pro的含量提高以及相关病程基因表达有关。To clarify the disease resistance and effect of tobacco induced by the protein elicitor isolated from mycelia of Colletotrichum destructivum,the dynamic activities of hydrogen peroxidase（ POD）,polyphenol oxidase（ PPO） and penylalanin ammonialyase（ PAL）,the content of proline（ Pro） and the expression change of pathogenesis-related genes were measured by using the spraying,friction inoculation methods and RT-PCR technique. The resistance effect to tobacco anthracnose were 58. 00%,48. 99%and 49. 65% after 3,5 and 7 days,respectively,and the resistance effect to tobacco powdery mildew were 83. 26%,80. 76% and 78. 60%,respectively. The protein elicitor could inhibit the replication and amplification of Tobacco mosaic virus in hosts. The activities of POD,PPO,PAL and the content of Pro of tobacco induced by the protein elicitor were increased significantly than the control. The transcription ofacid pathogenesis-related gene 1（ PR-1a）,basic pathogenesis-related gene 1（ PR-1b） and non-expresser of pathogenesis-related gene 1（ NPR1） were upregulated as well. The results indicated that the systemic acquired resistance in tobacco induced by protein elicitor might be related with the activity or content enhancement of POD,PPO,PAL,Pro and the induction of resistance-related gene expression.

关 键 词：毁灭炭疽菌 诱抗蛋白 诱导抗病性 防御酶 抗性相关基因 

分 类 号：S435.72[农业科学—农业昆虫与害虫防治]