中哈边境阿拉山口口岸输入性亚洲璃眼蜱的种类鉴定与分析  

Species identification of an imported tick sample of Hyalomma asiaticum asiaticum by genetic analysis at Alashankou port,China-Kazakhstan border

作　　者：徐军[1] 王安东[2] 戴莉[3] 杨杰[3] 罗丹[4] 徐建军[5] 李志远[2] 热依罕古丽 萨尼叶古丽 XU Jun WANG An-dong DAI Li YANG Jie LUO Dan XU Jian-jun LI Zhi-yuan RE Yi-han-gu-li SA Ni-ye-gu-li(Xinjiang Entry-Exit Inspection and Quarantine Authority of the PRC, Urumqi 830000, China College of Animal Science and Technology, Shihezi University, Shihezi $32000, China Alashankou Entry-Exit Inspection and Quarantine Authority of the PRC, Alashankou 833418, China College of Veterinary Medicine, Shihezi University, Shihezi 830052, China College of Veterinary Medicine , XND , Urumqi 830052,China)

机构地区：[1]新疆出入境检验检疫局,新疆乌鲁木齐832000 [2]石河子大学动物科技学院,新疆石河子832000 [3]阿拉山口出入境检验检疫局,新疆阿拉山口833418 [4]石河子大学医学院,新疆石河子832000 [5]新疆农业大学动物医学学院,新疆乌鲁木齐830052

出　　处：《中国兽医杂志》2016年第11期17-19,共3页Chinese Journal of Veterinary Medicine

基　　金：新疆维吾尔自治区自然科学基金(201442137-1);国家质检总局科技计划项目(2014IK239);公益性行业(农业)科研专项(201103008)

摘　　要：对1批从哈萨克斯坦进口羊毛中检获的蜱样品进行种类鉴定。应用16S rDNA基因和COI基因两条DNA条形码技术进行鉴定分析。结果 16S rDNA序列分析显示与新疆亚洲璃眼蜱(KC203351)同源性最接近(99%);COI基因序列分析显示与新疆伊犁亚洲璃眼蜱(KF527440)同源性最接近(99%)。结论:经DNA条形码比对,确定为亚洲璃眼蜱,应加强对外来医学媒介生物防控,严防"外来病"入侵。To identify the species of ticks in wool samples from Kazakhstan at Alashankou port,Xinjiang Uygur Autonomous Region,China.The 16 S rDNA and CO1 identification were carried out for the ticks,and sequence analysis was performed to establish the molecular phylogenetic tree.The 16 S rDNA and COI were clustered together with Hyalomma asiaticum asiaticum（accessionnumbers：KC203351,KF527440）;these strains had the highest homology with Hyalomma asiaticum asiaticum（99 %）.Based on DNA barcode comparison,the imported tick sample was identified as Hyalomma asiaticum asiaticum.We should strengthen the prevention and control of imported medical vectors,to prevent the ＂ Exotic Diseases＂.

关 键 词：亚洲璃眼蜱 种类鉴定 16S RNA COI 线粒体 阿拉山口口岸 

分 类 号：Q969[生物学—昆虫学]