淫羊藿苷对大鼠骨髓间充质干细胞迁移作用的影响  

Effects of Icariin on Migration of Mesenchymal Stem Cells of Rat Bone Marrow

作　　者：张黎声[1] 韩小晶[1] 罗志荣[1] 邵水金[1] 叶晓春[1] 牟芳芳[1] 国海东[1] 

机构地区：[1]上海中医药大学基础医学院,上海201203

出　　处：《中国中医药信息杂志》2017年第2期44-48,共5页Chinese Journal of Information on Traditional Chinese Medicine

基　　金：国家自然科学基金(81673729);上海市自然科学基金(16ZR1437300);上海市教育委员会科研创新项目(14YZ053)

摘　　要：目的探讨淫羊藿苷调控大鼠骨髓间充质干细胞(MSC)迁移作用机制。方法 CCK-8法检测细胞增殖。建立体外细胞缺糖缺氧模型,Hoechst33342染色观察细胞凋亡。Western blot检测淫羊藿苷处理后MSC表面基质细胞衍生因子l(SDF-1)受体趋化因子受体4(CXCR4)的蛋白表达。Transwell小室跨膜实验观察淫羊藿苷对MSC迁移的作用。结果 0.01、0.1、1μmol/L淫羊藿苷可明显促进MSC增殖,10μmol/L淫羊藿苷抑制MSC增殖。经体外缺糖缺氧处理后,0.1、1μmol/L淫羊藿苷可明显抑制MSC凋亡。淫羊藿苷可显著促进MSC CXCR4的蛋白表达,同时可提高MSC跨膜迁移的数量。加入CXCR4拮抗剂AMD3100后,各组间细胞迁移数量无明显差异。结论一定浓度淫羊藿苷可促进MSC的增殖、存活和迁移,SDF-1/CXCR4信号通路参与淫羊藿苷调控MSC迁移的作用。Objective To explore the mechanism of icariin mediating migration of mesenchymal stem cells（MSC） in rat bone marrow. Methods MSC proliferation was detected by CCK-8 test. Cell apoptosis was examined with Hoechst33342 staining after the establishment of cellular oxygen and glucose deprivation model. The protein expressions of CXCR4, the receptor of SDF-1, in the surface of MSC after stimulated by icariin were detected through Western blot. The migration of MSC was observed by Transwell chemotaxis assay. Results 0.01 μmol/L, 0.1 μmol/L and 1 μmol/L of icariin could significantly promote the proliferation of MSC, while 10 μmol/L of icariin inhibited the proliferation of MSC. After treatment of oxygen and glucose deprivation in vitro, 0.1 μmol/L and 1 μmol/L of icariin could inhibit the apoptosis of MSC. Icariin could not only improve the expression of CXCR4 in MSC, but also increase the number of transmembrane migrated MSC. After the addition of CXCR4 antagonist AMD3100, there was no significant difference in the number of cell migration among the different groups. Conclusion Icariin with appropriate concentration can promote the proliferation, survival and migration of MSC. SDF-1/CXCR4 signaling pathway is involved in the regulation of MSC migration by icariin.

关 键 词：淫羊藿苷 骨髓间充质干细胞 存活 迁移 信号通路 

分 类 号：R285.5[医药卫生—中药学]