β-榄香烯纳米聚合物胶束包封率、载药量及释药性测定  

Determination of Encapsulation Efficiency and Medicine Loading and Release of β-elemene in β-elemene-loaded Nano Polymeric Micelles

作　　者：王静[1] 李学涛[1] 袁子民[1] 

机构地区：[1]辽宁中医药大学,辽宁大连116600

出　　处：《中国中医药信息杂志》2017年第2期91-93,共3页Chinese Journal of Information on Traditional Chinese Medicine

基　　金：辽宁省科学技术计划(2015020703)

摘　　要：目的建立β-榄香烯纳米聚合物胶束包封率和载药量的RP-HPLC测定方法,并对其释药性进行考察。方法以二硬脂酰磷脂酰乙醇胺-聚乙二醇2000(DSPE-PEG2000)为载体材料制备载药胶束,采用石油醚萃取法测定包封率和载药量,采用透析法对其释药性进行研究。结果所制备的β-榄香烯纳米聚合物胶束平均包封率为89.47%,平均载药量为8.33%,体外释药缓慢。结论包封率和载药量的测定简便、准确,所制得的胶束具有缓释作用。Objective To establish a RP-HPLC method for the determination of encapsulation efficiency （EE） and medicine loading （ML） in β-elemene-loaded Nano Polymeric Micelles; To study its release characteristic in vitro. Methods DSPE-PEG2000 was used as carrier to prepare medicine-loaded micelles. EE and ML were determined by petroleum ether extraction method, and its release characteristic in vitro was studied by dialysis method. Results The average EE and ML of β-elemene-loaded Nano Polymeric Micelles were 89.47% and 8.33%, respectively. Its release characteristic was slow. Conclusion The method for EE and ML determination is simple and accurate, and the prepared micelles have the property of sustained release.

关 键 词：β-榄香烯纳米聚合物胶束 包封率 载药量 释药性 

分 类 号：R284.1[医药卫生—中药学]