褪黑素对带状疱疹后神经痛大鼠血清CD4^+ CD25^+调节性T细胞表达的影响  

The Influence of Melatonin on CD4^+ CD25^+ Regulatory T Cell Expression in Serum of Postherpetic Neuralgia Rats

作　　者：赵海玉[1] 邓云坤[2] 方开云[2] 杨淼[2] 

机构地区：[1]贵州医科大学,贵州贵阳550004 [2]贵州省人民医院麻醉科,贵州贵阳550002

出　　处：《贵州医科大学学报》2017年第1期52-55,63,共5页Journal of Guizhou Medical University

基　　金：贵州省科技计划(黔科合SY3122)

摘　　要：目的:观察褪黑素对带状疱疹后遗神经痛(PHN)大鼠CD4^+ CD25^+调节性T细胞表达的影响。方法:雄性SD大鼠60只,随机分为空白对照组(不做任何处理)、假手术组(仅暴露松果体)、PHN模型组(松果体切除后接种带状疱疹病毒)及治疗组(与PHN模型组一样造模,同时腹腔注射外源褪黑素);接种病毒30 d时进行机械痛觉评分,同时取大鼠外周血应用流式细胞术检测CD4^+ CD25^+调节性T细胞表达,应用RT-PCR技术检测Foxp3+基因表达,采用ELISA技术检测外周血TGF-β表达。结果:接种病毒30 d时,空白对照组与假手术组比较各项指标差异均无统计学意义(P>0.05);模型组和治疗组大鼠痛觉评分、外周血CD4^+ CD25^+调节性T细胞、Foxp3+基因和TGF-β表达均显著高于正常对照组(P<0.05);模型组各项指标均显著高于治疗组(P<0.05)。结论 :腹腔注射褪黑素可减轻带状疱疹病毒感染大鼠的PHN,其机制可能与下调CD4^+ CD25^+调节性T细胞表达改善机体免疫功能有关。Objective： To observe the influence of melatonin on β regulatory T cell ex- pression in postherpetie neuralgia （PHN） rats. Method ： Sixty male SD rats were randomly divided in- to 4 groups： blank control group （group B）, sham-operation group （group S）, PHN model group （group P）, and treatment group （group T）. Rats in group A were not dealt with specially. Conarium of rats in group B were exposed. Conarium of rats in group P were removed and the rats were inocula- ted with herpes zoster virus. Rats in group T were inject with exogenous menatonin intraperitoneally af- ter received operations of group P. In 30 day after virus inoculation, mechanical pain was graded, ex- pression of CD4＋CD25＋ regulatory T cells in rat peripheral blood （PB） was tested with flow cytome- try, Foxp3 ＋ gene expression detected with RT-PCR technique, and PB TGF-13 expression determined using ELLISA technology. Result： All of the indicators were not significantly different between groups B and S（P 〉 0.05）. The pain scores, expressions of PB CD4＋CD25＋ regulatory T cells, Foxp3 ＋ gene, and TGF-β were significantly higher in groups P and T than in group B （ P 〈 0.05 ） , and were markedly higher in group P than in group T. Conclusion： Peritoneal cavity injection of melatonincould relieve PHN in herpes zoster virus infected rats. The mechanism might relate with the decrease of expression of CD4＋CD25＋ regulatory T cells and the improvement of immunity.

关 键 词：褪黑素 带状疱疹 神经痛CD4+CD25+调节性T细胞 大鼠 Sprague—Dawley 

分 类 号：R512.92[医药卫生—内科学]