BMSCs与PHBHHx骨支架材料生物相容性体外实验研究  

Experimental Study on Biocompatibility of BMSCs and PHBHHx Scaffold Materials in vitro

作　　者：周诚[1] 李竹[2] 胡瑞洁[2] 刘日光[3] 叶川[3] 贾湘谦[1] 吴昌林[1] 韩子翼[1] 

机构地区：[1]贵阳市第一人民医院,贵州贵阳550002 [2]贵州医科大学,贵州贵阳550004 [3]贵州医科大学附院,贵州贵阳550004

出　　处：《贵州医科大学学报》2017年第1期56-59,63,共5页Journal of Guizhou Medical University

基　　金：贵阳市科技局资助项目2014科技类计划[筑科合同(2014)100125号]

摘　　要：目的:研究人骨髓间充质干细胞(BMSCs)与3-羟基丁酸酯-3-羟基己酸酯共聚物(PHBHHx)的生物相容性。方法:将PHBHHx(实验组)及明胶海绵(对照组)与第7代BMSCs复合培养7 d,通过光镜和扫描电镜分别观察BMSCs的生长及分布情况。结果:联合培养7 d,在显微镜下均可见两组材料周围有大量细胞生长,而材料内部及表面的细胞生长情况不能观察;电镜下见PHBHHx材料表面及孔隙内均有大量细胞附着,细胞相互连接融合成片,充满材料孔隙与表面,细胞表面可见大量的微绒毛,表示细胞生长状态良好,而明胶海绵上未见明显细胞生长。结论:PHBHHx具有良好的BMSCs相容性,可以作为一种新型的生物材料应用于组织工程。Objective： To study the biocompatibility of bone marrow mesenchymal stem cells （BM- SCs） and 3-hydroxybutyrate-co-3-hydroxyhexanoate copolyesters （PHBHHx）. Methods： The PHBH- Hx （experimental group） and gelatin sponge （control group） were cultured with BMSCs of the seventh generation for 7 days, and the growth and distribution condition of BMSCs were observed by light mi- croscopy and scanning electron microscopy. Results ： After cultured for 7 days, under the microscope there were a large number of cells around the two materials, while inside and outside the material the cell growth cannot be observed ; under electron microscope, attachment of a large number of cells were visible on the surface and in the pores of PHBHHx material, cells connected and were full of surface and pores, There are a lot of microvilli on cell surface, which showed cells in good condition, but no obvious cell growth were found in gelatin sponge. Conclusion： PHBHHx membrane has good BMSCs compatibility, and it can be used as a new biological materials in tissue engineering.

关 键 词：骨髓 间充质干细胞 组织工程 3-羟基丁酸酯-3-羟基己酸酯共聚物 明胶海绵 生物相容性 

分 类 号：R318.17[医药卫生—生物医学工程]