鹅不食草药材的质量研究  

Quality Study on Centipeda Minima

作　　者：张自强[1,2,3] 田磊[2] 邱斌 杨叶婷[1] 周志宏[1] 程永现[2] 张雯洁[3] 

机构地区：[1]云南中医学院中药学院,云南昆明650500 [2]中国科学院昆明植物研究所,植物化学与西部植物资源持续利用国家重点实验室,云南昆明650201 [3]云南省食品药品检验所中药室,云南昆明650011 [4]云南省药物研究所天然药物资源研究室,云南昆明650111

出　　处：《中国医院用药评价与分析》2016年第11期1462-1466,共5页Evaluation and Analysis of Drug-use in Hospitals of China

基　　金：中国科学院重点部署项目(No.KSZD-EW-Z-004-03-3)

摘　　要：目的：参照《中华人民共和国药典》（2015年版）附录相关方法,对来自全国多批鹅不食草药材进行质量标准的提高,以完善《中华人民共和国药典》中鹅不食草药材的质量标准。方法：对鹅不食草药材的性状、显微特征进行研究;对其水分、浸出物进行测定;以对照药材和与药材功效相关的主要成分6-氧-当归酰多梗白菜菊素（6-O-angeloylplenolin,6-OAP）为对照,采用薄层色谱方法进行定性鉴别;以与药材功效相关的主要成分6-OAP为指标,采用高效液相色谱法进行含量测定。结果：鹅不食草的显微特征明显;薄层色谱斑点清晰;6-OAP在鹅不食草中在0.200 75-8.030 00μg区间呈良好的线性关系（r=0.999 9）,平均加样回收率为103%,6-OAP的质量分数为0.13%-0.32%（平均值为0.21%）。结论：本研究所建立的方法简单易行、准确可靠,可用于鹅不食草的质量控制和《中华人民共和国药典》标准提高。OBJECTIVE： By reference to the relevant methods of appendix of Chinese Pharmacopoeia,the quality standard of Centipeda minima from all over the country would be improved,so as to perfect the quality standard of Centipeda minima in Chinese Pharmacopoeia. METHODS： The character and microscopic features of Centipeda minima were studied,and the water content and extract yield were determined. By reference to the standard crude drugs and the major components of 6-O-angeloylplenolin（ 6-OAP） that associated with medicinal efficacy,TLC method was adopted to conduct the qualitative discrimination; to set 6-OAP as indicators,HPLC method was adopted to conduct the content determination. RESULTS： The microscopic features of Centipeda minima were significant; and TLC spots were clear,the linear ranges of 6-O-angeloylplenolin was 0. 200 75-8. 030 00 μg（ r = 0. 999 9）. The average recovery was 103%. The quantity of 6-O-angeloylplenolin was 0. 13%-0. 32%（ average 0. 21%）. CONCLUSIONS： The method developed in this study are simple and easy to conduct,which could be used to better the quality control of Centipeda minima.

关 键 词：质量标准 鹅不食草 含量测定 6-氧-当归酰多梗白菜菊素 

分 类 号：R927[医药卫生—药学]