中药复方拮抗小鼠运动应激时血中儿茶酚胺升高  

Inhibitory effect of Chinese complex prescription medicine on elevated levels of catecholamines in mice plasma during motion stress

作　　者：白海波 万海同 宋子荣 李波 

机构地区：[1]养生堂天然药物研究所,浙江杭州310007 [2]浙江中医学院,浙江杭州310053

出　　处：《中草药》2002年第8期716-718,共3页Chinese Traditional and Herbal Drugs

基　　金：国家自然科学基金项目 (3 0 0 70 941)

摘　　要：目的　观察人参、麦冬、葛根等提取物以及速效救心丸对小鼠运动应激时血中儿茶酚胺水平的影响。方法健康 ICR小鼠置跑步机上跑步 10 m in造成运动应激 ,用高效液相色谱 -电化学联合检测法测定运动应激动物血浆中儿茶酚胺水平。结果　运动应激小鼠血浆中去甲肾上腺素和肾上腺素浓度显著增加 (P<0 .0 1) ,小鼠 ig人参、麦冬、葛根等提取物以及速效救心丸 1h后再运动应激可拮抗血浆中去甲肾上腺素升高 (P<0 .0 1)。结论　小鼠运动应激可使交感神经激活 ,导致血中去甲肾上腺素释放增加 ,人参、麦冬、葛根提取物和速效救心丸可拮抗之。Object To investigate the effect of Chinese complex prescription medicine, which included Radix Ginseng, Radix Ophiopogonis, Radix Puerariae extracts, and SUXIAO JIUXIN PILL  on catecholamine levels in stress mice plasma while the mice were forced running on treadmill. Methods Motion stress was caused by forcing ICR mice to run on treadmill for 10 min. Based on reversed phase, ion pair HPLC combined with electrochemical detection, a procedure with minimal plasma sample pretreatment was developed for the determination of the catecholamines level in plasma samples. Results The plasma norepinephrine and epinephrine concentration increased markedly (P<0 01) in stress mice compared with the control one. But the norepinephrine increase was inhibited (P<0 01) in taking the blood samples in motion stress after 1 h of ig the mice with Radix Ginseng, Radix Ophiopogonis, Radix Puerariae extracts and SUXIAO JIUXIN PILL. Conclusion Motion stress of mice activates the sympathetic nerve and leads to increase the release of norepinephrine in blood, whereas the Radix Ginseng, Radix Ophiopogonis, Radix Puerariae extracts and SUXIAO JIUXIN PILL used in the present study can inhibit this increasing release.

关 键 词：中药复方 小鼠 运动应激 儿茶酚胺 速效救心丸 高效液相色谱-电化学检测 交感神经活性 

分 类 号：R285.5[医药卫生—中药学]