IL-17A抗体抑制变应性鼻炎鼠模型中IL-17A及RORγt并升高Foxp3 mRNA表达  

IL-17A antibody inhibited the IL-17A and RORγt and increased Foxp3 mRNA expression in allergic rhinitis mice

作　　者：顾兆伟[1] 王韫秀[2] 赵鹤[1] 曹志伟[1] 

机构地区：[1]中国医科大学附属盛京医院耳鼻咽喉科,沈阳110004 [2]中国医科大学附属盛京医院医疗保险工作部,沈阳110004

出　　处：《临床与病理杂志》2016年第12期1986-1990,共5页Journal of Clinical and Pathological Research

基　　金：国家自然科学基金(81200730);辽宁省自然科学基金项目(2014021047);辽宁省教育厅科学研究项目(LK201601);沈阳市科技局项目(F15-199-1-41)~~

摘　　要：目的:探讨IL-17A抗体对小鼠变应性鼻炎鼻黏膜中IL-17A、RORγt、Foxp3 mRNA水平的影响及意义。方法:以卵清蛋白致敏的Balb/c小鼠变应性鼻炎模型作为实验组,同期以生理盐水替代作为对照组,使用IL-17A抗体治疗作为治疗组。实时定量PCR方法检测三组小鼠鼻黏膜中IL-17A、RORγt、Foxp3mRNA的含量。结果:实验组中RORγt以及IL-17AmRNA水平均较对照组升高(P<0.05),治疗组中RORγt以及IL-17AmRNA的含量均低于实验组(P<0.05)。而实验组中Foxp3 mRNA水平较对照组下降(P<0.0 5),治疗组中Foxp3 mRNA的含量高于实验组(P<0.0 5)。结论:IL-17A抗体抑制变应性鼻炎鼠模型中IL-17A及RORγt mRNA水平并升高Foxp3 mRNA表达水平。Objective： To investigate the effect of IL-17A antibody on IL-17A, RORγt, Foxp3 mRNA in nasal mucosa of allergic rhinitis mice.Methods： BALB/C mice allergic rhinitis model was constructed with ovalbumin sensitization as the experimental group （AR group）, saline was used for control group and IL-17A antibody was used for therapy group. IL-17A, RORγt, Foxp3 mRNA in nasal mucosa of the three groups were measured by using real-time PCR.Results： RORγt and IL-17A mRNA in AR group were signiifcantly higher than those in control group （P〈0.05）. hTe expression of RORγt and IL-17A mRNA in therapy group was lower than those in AR group （P〈0.05）. hTe level of Foxp3 mRNA in AR group was lower than that in the control group （P〈0.05）, and the expression of Foxp3 mRNA in therapy group was higher than that in AR group （P〈0.05）.Conclusion： IL-17A antibody inhibited the IL-17A and RORγt mRNA levels and increased Foxp3 mRNA expression in allergic rhinitis mice. hTe results of this study will help to understand the pathogenesis of allergic rhinitis, which provides a theoretical basis and a new way in the targeted therapy for allergic rhinitis.

关 键 词：变应性鼻炎 白细胞介素17A RORΓT FOXP3 

分 类 号：R765.21[医药卫生—耳鼻咽喉科] R-332[医药卫生—临床医学]